Isolation, growth and characteristics of human ovarian surface epithelium
Isolation, growth and characteristics of human ovarian surface epithelium
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人卵巢表面上皮的分离、生长及特性
DOI：
10.1007/bf00197394
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发表时间：
2004
期刊：
影响因子：
3.5
通讯作者：
H. Okamura
中科院分区：
文献类型：
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作者：
M. Nakamura;H. Katabuchi;T. Ohba;Y. Fukumatsu;H. Okamura
卵巢表面上皮（OSE）是卵巢表面上皮间质肿瘤和卵巢子宫内膜异位症发病机制中的关键组织，是常见的妇科疾病。尽管这些疾病的发病率很高，但OSE的体外实验研究很少，因此我们使用刮擦法和酶法分离人OSE并研究其体外特性。使用19个正常卵巢。卵巢在1型胶原酶溶液（300 U/ml）中孵育40 min后，用手术刀片轻轻刮除表面细胞。将在培养基199中用5%牛血清白蛋白进行单位重力沉降后获得的成簇细胞在含有15%胎牛血清的培养基199中培养。单个卵巢中的活细胞数为0.1 - 2.7×106。细胞的生长从单一细胞的同质群体开始，并且细胞群体倍增时间在7和10天之间。13-20天后形成汇合的单层，并传代培养1 - 3次。单层细胞多呈鹅卵石样外观，也可见梭形或多边形细胞。经细胞化学、免疫细胞化学、扫描电镜和透射电镜观察，短期培养的细胞具有间皮OSE细胞的特征。这种实验方法有效地提供了培养的人OSE，其可用于研究病理生物学和致癌作用。
The ovarian surface epithelium (OSE) is a key tissue in the pathogenesis of ovarian surface epithelial-stromal tumours and ovarian endometriosis, commonly encountered gynaecological diseases. Despite the high incidence of these diseases, experimental in vitro studies of OSE are few and so we used the scraping method with an enzymatic procedure to isolate human OSE and studied its characteristics in vitro. Nineteen normal ovaries were used. After incubation of the ovary for 40 min in collagenase type 1 solution (300 U/ml), the surface cells were removed by gentle scraping with a surgical blade. Cells obtained as a cluster after unit gravity sedimentation with 5% bovine serum albumin in medium 199 were cultured in medium 199 containing 15% fetal bovine serum. The viable cell number in a single ovary was 0.1−2.7×106. The outgrowth of cells started from a homogeneous population of single cells, and the cell population doubling time was between 7 and 10 days. Confluent monolayers were formed after 13–20 days and subcultured from one to three times. The monolayers mostly had a cobblestone appearance, and fusiform or polygonal cells were also observed. By cytochemistry, immunocytochemistry and scanning and transmission electron microscopy, the cells were shown to have characteristics of mesothelial OSE cells in short-term culture. This experimental approach was efficient in providing cultured human OSE, which can be utilized to investigate pathobiology and carcinogenesis.
人间皮和间皮瘤在体内和培养物中简单上皮角蛋白和波形蛋白的共表达。
影响因子：
11.2
作者：
LaRocca,PJ;Rheinwald,JG
通讯作者：
Rheinwald,JG
兔卵巢间皮（表面上皮）的分离和超微结构。
DOI：
--
发表时间：
1984
期刊：
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists
影响因子：
--
作者：
Nicosia,SV;Johnson,JH;Streibel,EJ
通讯作者：
Streibel,EJ