ChABC-loaded PLGA nanoparticles: A comprehensive study on biocompatibility, functional recovery, and axonal regeneration in animal model of spinal cord injury
ChABC-loaded PLGA nanoparticles: A comprehensive study on biocompatibility, functional recovery, and axonal regeneration in animal model of spinal cord injury
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DOI：
10.1016/j.ijpharm.2020.119037
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发表时间：
2020-03-15
影响因子：
5.8
通讯作者：
Omidian, Hamid
中科院分区：
文献类型：
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作者：
Azizi, Monireh;Farahmandghavi, Farhid;Omidian, Hamid
脊髓损伤（SCI）是导致身体残疾的主要原因之一。在本研究中，制备了含有ChABC酶的球形PLGA纳米颗粒（NPs），并对其进行了充分的表征。NPs用于大鼠挫伤脊髓，以评估功能改善和疤痕消化。将23只成年雄性Wistar大鼠（275 +/- 25 g）分为对照组、假药组、空白处理组和chabc处理组。在整个调查过程中，获得了所有组的BBB分数。最后解剖动物损伤切片，采用Luxol快蓝和Bielschowsky进行组织学研究。MTT实验证实了NPs对嗅鞘细胞（OECs）的生物相容性和无毒作用。流式细胞术显示p75(+)/GFAP（+）的OECs纯度约为87.9 +/- 2.4%。对照组和空白处理组的动物BBB评分明显低于chabc处理颗粒组。组织学结果证实了损伤部位的诱导挫伤模型。在处理组中观察到髓磷脂，特别是当使用装载chabc的纳米颗粒时。免疫组化结果显示，载chabc颗粒处理的动物瘢痕胶质细胞降解。根据这项研究，负载颗粒有可能成为脊髓修复、功能恢复和轴突再生的合适候选材料。
Spinal Cord Injury (SCI) is one of the leading causes of physical disability. In this study, spherical PLGA nanoparticles (NPs) containing ChABC enzyme were manufactured and fully characterized for SCI therapy. The NPs were used in the rat's contused spinal cord to assess the functional improvement and scar digestion. Twenty-three adult male Wistar rats (275 +/- 25 g) were assigned into four groups of control, sham, blank-treated particle, and ChABC-treated particle. Throughout the survey, the BBB scores were obtained for all the groups. Finally, the injured sections of animals were dissected, and histological studies were conducted using Luxol fast blue and Bielschowsky. The biocompatibility and non-toxicity effects of the NPs on olfactory ensheathing cells (OECs) were confirmed by the MTT test. The flow-cytometry revealed the purity of cultured OECs with p75(+)/GFAP(+) at around 87.9 +/- 2.4%. Animals in the control and the blank-treated groups exhibited significantly lower BBB scores compared with the ChABC-treated particle group. Histological results confirmed the induced contusion models in the injured site. Myelin was observed in the treated groups, especially when the ChABC-loaded nanoparticles were utilized. The immunohistochemistry results indicated the scar glial degradation in animals treated by the ChABC-loaded particles. According to this study, the loaded particles can potentially serve as a suitable candidate for spinal cord repair, functional recovery and axonal regeneration.