Expression of the human erythrocyte glucose transporter Glut1 in cutaneous neoplasia
Expression of the human erythrocyte glucose transporter Glut1 in cutaneous neoplasia
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DOI：
10.1016/s0190-9622(97)70174-9
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发表时间：
1997-10-01
影响因子：
13.8
通讯作者：
Younes, M
中科院分区：
文献类型：
--
作者：
Baer, SC;Casaubon, L;Younes, M
目的：研究人红细胞葡萄糖转运蛋白(Glut1)在皮肤肿瘤中的表达。方法：采用多克隆抗Glut1抗体(MYM)和ABC免疫组织化学技术，检测浸润性鳞癌(SCCs)、原位鳞癌、基底细胞癌(BCC)、黑色素瘤、光化性角化病(AKs)、脂溢性角化病、常见获得性新生血管和瘢痕疙瘩中Glut1的表达。结果：15例皮肤鳞状细胞癌中，14例(93%)为鳞状细胞癌、原位鳞状细胞癌、基底细胞癌、黑色素瘤、光化性角化病、脂溢性角化病、常见获得性新生血管和瘢痕并伴有再生的表皮上皮增生性病变。15例AK中有13例(87%)Glut1呈强膜性染色。Glut1在15例瘢痕中有8例(53%)在表皮中表达，但在任何基底细胞癌中均未检测到，甚至在局限性角化区和鳞状化生区域也未检测到。Glut1在黑色素瘤和脂溢性角化病中缺乏反应性。结论：皮肤病变中Glut1的表达强烈提示鳞状细胞类型的增殖性病变。
Background: The increased glucose uptake seen in cancer cells correlates with the expression of human erythrocyte glucose transporter (Glut1) protein in certain human malignancies.Objective: Our purpose was to determine Glut1 expression in cutaneous neoplasms.Methods: A polyclonal anti-Glut1 antibody (MYM) and a standard ABC immunoperoxidase technique were used to determine Glut1 expression in invasive squamous cell carcinomas (SCCs), SCC in situ, basal cell carcinomas (BCCs), melanomas, actinic keratoses (AKs), seborrheic keratoses, common acquired nevi, and scars with regenerative epidermal hyperplasia.Results: All of the cases of SCC in situ, 14 of 15 (93%) of the SCC, and 13 of 15 AKs (87%) showed intense membranous staining for Glut1. Glut1 staining was present in the epidermis of 8 of 15 scars (53%) but was not detected in any BCC, even in areas of focal keratinization and squamous metaplasia. Glut1 reactivity was absent in the melanomas and seborrheic keratoses.Conclusion: Glut1 expression in a cutaneous lesion strongly suggests a proliferative lesion of the squamous cell type.