Structural differences between two types of basidiomycete septal pore caps
Structural differences between two types of basidiomycete septal pore caps
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DOI：
10.1099/00221287-144-7-1721
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发表时间：
1998-07-01
期刊：
影响因子：
--
通讯作者：
Boekhout, T
中科院分区：
文献类型：
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作者：
Müller, WH;Montijn, RC;Boekhout, T
Trichosporon sporotrichoides CBS 8245 的隔膜孔帽 (SPC) 呈囊状管状，与平管状内质网 (ER) 连接，用锌/碘/四氧化锇染色致密。急诊室也是如此。裂褶菌 CBS 340.81 的 SPC 更复杂，直径约为 600 nm，穿孔直径为 80-120 nm，锌/碘/四氧化锇染色密度低于 ER。在高压冷冻和冷冻替代的 T.sporotrichoides 菌丝中，ER 与多里孔隔膜平行存在，电子致密材料出现在隔膜孔通道对面； SPC 很少显示出光滑的囊泡管膜，表明这是 SPC 的短暂功能。 S. commune 的 SPC 具有光滑的外膜和内膜，其包围具有栅栏状子结构的基质。 5. commune的SPC内表面覆盖一层薄薄的电子致密材料，珠状丝状结构将SPC和孔堵塞材料连接起来。这些丝状结构可以维持 SPC 的细胞内位置，并可能在堵塞隔膜孔通道中发挥作用。 T,sporotrichoides 的间隔孔肿胀含有比间隔更多的 1,6-β-葡聚糖，细胞内葡聚糖也存在于间隔孔通道附近。 T.sporotrichoides 中的这种胞质 1,6-β-葡聚糖可以作为基质将 SPC 的管状膜结构保持在一起。相反，在 5. commune 的 SPC 和孔闭塞材料中未观察到 1,6-β-葡聚糖，并且该物种的菌丝隔膜显示出的 1,6-β-葡聚糖标记少于隔膜肿胀。从简单类型到更复杂类型的 SPC 的进化转变可能导致需要不同的成分来维持形态完整性和细胞生物学功能。
The septal pore cap (SPC) of Trichosporon sporotrichoides CBS 8245 is vesicular-tubular, connected with flat-tubular endoplasmic reticulum (ER), and stains densely with zinc/iodine/osmium tetroxide. as does the ER. The SPC of Schizophyllum commune CBS 340.81 is more complex, about 600 nm in diameter, with perforations of 80-120 nm diameter, and stains less densely with zinc/iodine/osmium tetroxide than the ER. In high-pressure frozen and freeze-substituted hyphae of T.sporotrichoides the ER is present parallel to the dolipore septa, and electron-dense material occurs opposite the septal pore channel; the SPC rarely showed smooth vesicular-tubular membranes, suggesting that this is an ephemeral function of the SPC. The SPC of S. commune has a smooth outer and inner membrane, which enclose a matrix with a palisade-like substructure. A thin layer of electron-dense material covers the inner surface of the SPC of 5. commune, from which beaded filamentous structures connect the SPC and the pore-occluding material. These filamentous structures may maintain the intracellular position of the SPC and possibly play a role in plugging the septal pore channel. The septal pore swellings of T,sporotrichoides contain more 1,6-beta-glucan than the septum, and intracellular glucans are also present near the septal pore channel. This cytosolic 1,6-beta-glucan in T.sporotrichoides may serve as a matrix to keep the tubular membranous structures of the SPC together. In contrast 1,6-beta-glucan is not observed in the SPC and in the pore-occluding material of 5. commune, and hyphal septa of this species show less labelling of 1,6-beta-glucan than the septal swelling. The evolutionary transition from simple to more complex types of SPCs may have resulted in a requirement for different components to maintain the morphological integrity and cell biological function.