Natural genetic recombination between co-circulating heterotypic enteroviruses
Natural genetic recombination between co-circulating heterotypic enteroviruses
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DOI：
10.1099/0022-1317-83-9-2193
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发表时间：
2002-09-01
影响因子：
3.8
通讯作者：
Crainic, R
中科院分区：
文献类型：
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作者：
Oprisan, G;Combiescu, M;Crainic, R
脊髓灰质炎病毒的自然重组是一种常见现象。在实践中，每当不同基因型有机会感染同一个体时，具有重组基因组的高比例病毒被排出。为了确定脊髓灰质炎病毒以外的肠道病毒是否可以自然地产生具有重组基因组的活病毒体，我们研究了与无菌性脑膜炎大爆发相关的埃可病毒血清型的病毒基因组的两个远距离区域-VP 1编码区和3D聚合酶编码区的分子特征。将两个基因组区域（VP 1的300 nt和3D聚合酶的520 nt）中的9个流行株[属于埃可病毒血清型4（E4）、7（E7）和30（E30）]的核苷酸序列与原型和野外株进行比较，并从比对中生成系统发育树。在VP 1区，三种流行血清型中的每一种都与同型原型菌株聚簇，而在3D聚合酶区，E7和E30聚为一个簇，远离两个相应的原型菌株。这表明这两种E7和E30菌株中的一种通过与另一种重组而进化，或者两者都从共同的祖先获得了3D聚合酶编码区。我们的研究结果表明，当多种流行毒株同时流行时，不同埃可病毒血清型之间的这种遗传重组是可能的。
Natural recombination in poliovirus is a frequent phenomenon. In practice, whenever different genotypes have the opportunity to infect the same individual, a high proportion of viruses with recombinant genomes are excreted. To determine whether enteroviruses other than poliovirus can naturally produce viable virions with recombinant genomes, we studied the molecular features of two distant regions of the viral genomes - the VP1 coding region and the 3D polymerase coding region - of the echovirus serotypes associated with a large outbreak of aseptic meningitis. Nucleotide sequences of nine epidemic strains [belonging to echovirus serotypes 4 (E4), 7 (E7) and 30 (E30)] in the two genomic regions (300 nt of VP1 and 520 nt of 3D polymerase) were compared to prototype and field strains, and phylogenetic trees were generated from alignments. In the VP1 region, each of the three epidemic serotypes clustered with the homotypic prototype strain, whereas in the 3D polymerase region, E7 and E30 grouped as a single cluster, distant from the two corresponding prototype strains. This suggests that one of these two E7 and E30 strains has evolved through recombination with the other or that both have acquired the 3D polymerase coding region from a common ancestor. Our results suggest that such genetic recombinations between different echovirus serotypes are possible when multiple epidemic strains are circulating simultaneously.