A jumbo phage infecting the phytopathogen Ralstonia solanacearum defines a new lineage of the Myoviridae family
A jumbo phage infecting the phytopathogen Ralstonia solanacearum defines a new lineage of the Myoviridae family
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DOI：
10.1016/j.virol.2009.11.043
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发表时间：
2010-03-01
期刊：
影响因子：
3.7
通讯作者：
Ogata, Hiroyuki
中科院分区：
文献类型：
--
作者：
Yamada, Takashi;Satoh, Souichi;Ogata, Hiroyuki
phi RSL1 是一种巨型肌病毒，可稳定地裂解性感染植物病原菌青枯菌。在这项研究中，我们研究了 phi RSL1 的感染周期，并提供了该噬菌体的基因组、蛋白质组和转录组视图。其 231 kbp 的基因组序列显示，许多基因在当前数据库中缺乏可检测的同源物，并且与之前研究的噬菌体基因组有很大不同。除了这些孤儿蛋白之外，phi RSL1 还被发现编码几种已知病毒中独特的酶。这些包括用于 NAD(+) 挽救途径的酶以及用于脂质、碳水化合物和高亚精胺生物合成途径的酶。发现一种类似几丁质酶的蛋白质是一种潜在的裂解酶。我们的蛋白质组学分析表明 phi RSL1 病毒粒子包含至少 25 种不同的蛋白质。我们通过 N 端测序鉴定了其中的 6 个蛋白，包括尾鞘蛋白和拓扑异构酶 IB。基于 DNA 微阵列分析，我们确定了两种转录模式。 (C) 2009 Elsevier Inc. 保留所有权利。
phi RSL1 is a jumbo myovirus stably and lytically infecting the phytopathogenic bacterium Ralstonia solanacearum. In this study, we investigate the infection cycle of phi RSL1 and provide a genomic, proteomic and transcriptomic view of this phage. Its 231-kbp genome sequence showed many genes lacking detectable homologs in the current databases and was vastly different from previously studied phage genomes. In addition to these orphan proteins, phi RSL1 was found to encode several enzymes that are unique among known viruses. These include enzymes for the salvage pathway of NAD(+) and for the biosynthetic pathways of lipid, carbohydrate and homospermidine. A chitinase-like protein was found to be a potential lysis enzyme. Our proteomics analysis suggests that phi RSL1 virions contain at least 25 distinct proteins. We identified six of them including a tail sheath protein and a topoisomerase IB by N-terminal sequencing. Based on a DNA microarray analysis, we identified two transcription patterns. (C) 2009 Elsevier Inc. All rights reserved.