ACTIVATION OF HELIX-LOOP-HELIX PROTEINS ID1, ID2 AND ID3 DURING NEURAL DIFFERENTIATION
ACTIVATION OF HELIX-LOOP-HELIX PROTEINS ID1, ID2 AND ID3 DURING NEURAL DIFFERENTIATION
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DOI：
10.1006/bbrc.1994.1380
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发表时间：
1994-03-30
影响因子：
3.1
通讯作者：
TODOKORO, K
中科院分区：
文献类型：
--
作者：
NAGATA, Y;TODOKORO, K
ID是一种含有螺旋-环-螺旋(HLH)基序的核因子。已有研究表明，ID是一种细胞分化抑制因子，其基因表达在细胞分化过程中下调。我们已经鉴定了从神经生长因子(NGF)刺激的PC12细胞中分离出来的三个ID相关的cDNA，Id1，Id2和Id3。结构分析表明，这三个ID在HLH基序附近和内部都含有可能的cAMP依赖的激酶、酪蛋白激酶II和蛋白激酶C的磷酸化位点。与以前的报道相反，Northern印迹分析表明，NGF诱导了所有三个ID基因转录的快速和瞬时增加。此外，大鼠胚胎的原位杂交显示，这三个ID基因都在神经前体细胞中高表达，而不是在分化的神经细胞中表达。这些结果表明，ID家族成员可能作为即刻早期基因产物发挥作用，并且ID家族的表达可能在神经分化的早期阶段发挥重要作用。(C)1994年学术出版社。
Id is a nuclear factor containing a helix-loop-helix (HLH) motif. It has been reported that Id functions as an inhibitor of cell differentiation and its gene expression is down-regulated during cell differentiation. We have characterized three Id-related cDNAs, Id1, Id2 and Id3, isolated from nerve growth factor (NGF)-stimulated PC12 cells. Structural analysis revealed that all three Id's contain putative phosphorylation sites for cyclic AMP-dependent kinase, casein kinase II and protein kinase C near and inside the HLH motifs. In contradiction to the previous reports, Northern blot analysis revealed that NGF induces rapid and transient increase of all three Id gene transcriptions. Furthermore, in situ hybridization of rat embryo showed that all three Id genes are highly expressed in neural precursors rather than differentiated neural cells. These results indicate that Id family members may function as immediate-early gene products, and that the expression of the Id family may play an important role in the early stage of neural differentiation. (C) 1994 Academic Press, Inc.