Dried Blood Spots Stored at Room Temperature Should Not Be Used for HIV Incidence Testing
Dried Blood Spots Stored at Room Temperature Should Not Be Used for HIV Incidence Testing
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DOI：
10.1089/aid.2018.0138
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发表时间：
2018-10-17
影响因子：
1.5
通讯作者：
Laeyendecker, Oliver
中科院分区：
文献类型：
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作者：
Eisenberg, Anna L.;Patel, Eshan U.;Laeyendecker, Oliver
限制性抗原（LAg）-亲合力测定是一种用于横断面HIV发病率检测的血清学测定。我们比较了使用室温（18 ℃-25 ℃）下储存或-80 ℃下冷冻储存的干血斑（DBS）样本进行的LAg亲合力测定获得的结果与从匹配血浆样本获得的结果。在南非的HIV预防试验网络（HPTN）068试验（2012-2014）中采集了匹配的DBS和血浆样本（306份配对样本）。测试前，DBS储存在室温下。2016年从HIV发病率测定评价和性能联盟（CEPHIA）收集了匹配的DBS和血浆样本（100对样本），并储存在-80 ℃下。所有DBS测试均于2017年进行。比较了匹配DBS和血浆样本之间的归一化光密度（ODn）差异。对于在室温下储存的DBS样本（HPTN 068），血浆与DBS的ODn值的平均差异为1.49（95%置信区间[CI]：1.36-1.62）。相比之下，当DBS样本储存在-80 ℃（CEPHIA）时，血浆与DBS的ODn值的平均差异为-0.22（95% CI：-0.32至-0.13）。室温下储存的DBS样本不应用于LAg亲合力测定的横断面HIV发病率检测。
The limiting antigen (LAg)-avidity assay is a serologic assay used for cross-sectional HIV incidence testing. We compared the results obtained with the LAg-avidity assay using dried blood spot (DBS) samples stored at room temperature (18 degrees C-25 degrees C) or stored frozen at -80 degrees C with results obtained from matched plasma samples. Matched DBS and plasma samples (306 paired samples) were collected in the HIV Prevention Trials Network (HPTN) 068 trial in South Africa (2012-2014). The DBS were stored at room temperature before testing. Matched DBS and plasma samples (100 paired samples) from the Consortium for the Evaluation and Performance of HIV Incidence Assays (CEPHIA) were collected in 2016 and were stored at -80 degrees C. All DBS testing was performed in 2017. Differences in normalized optical density (ODn) were compared between matched DBS and plasma samples. For DBS samples stored at room temperature (HPTN 068), the average difference in ODn values for plasma versus DBS was 1.49 (95% confidence intervals [CI]: 1.36-1.62). In contrast, when DBS samples were stored at -80 degrees C (CEPHIA), the average difference in ODn values for plasma versus DBS was -0.22 (95% CI: -0.32 to -0.13). DBS samples stored at room temperature should not be used for cross-sectional HIV incidence testing with the LAg-avidity assay.