Adaptation to eccentric exercise: effect on CD64 and CD11b/CD18 expression.

Adaptation to eccentric exercise: effect on CD64 and CD11b/CD18 expression.
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适应离心运动:对 CD64 和 CD11b/CD18 表达的影响。

DOI:
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发表时间:
1996
影响因子:
3.3
通讯作者:
Thomas A. Naglieri
Thomas A. Naglieri
中科院分区:
医学2区
文献类型:
--
作者:
F. Pizza;Bruce H. Davis;S. D. Henrickson;Joel B. Mitchell;J. F. Pace;N. Bigelow;P. C. DiLauro;Thomas A. Naglieri

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本研究的主要目的是检测两组(B1和B2)偏心运动后循环中性粒细胞和单核细胞及其质膜CD64、CD11b和CD18的表达。受试者(n = 10)进行25次前臂屈肌强制延长收缩,两次间隔3周。在运动前和恢复后1.5、6、12、24、48、72和96小时采集血样。CD64、CD11b和CD18的表达通过直接免疫荧光测定,并作为中性粒细胞和单核细胞活化的指标。肌酸激酶活性(B1 = 1,390, B2 = 108 U/l)、肌红蛋白(B1 = 163, B2 = 41, ng/dl)、肌肉酸痛和压痛均高于B2 (P < 0.01)。6、12、96 h时,B1组中性粒细胞高于B2组(P < 0.05)。CD11b在中性粒细胞上的表达在72 h时B1比B2高2.7倍。在72和96 h时,中性粒细胞上CD64的表达分别是B1和B2的1.4倍和1.9倍。在72和96 h时,B1细胞的CD18和CD64在单核细胞上的表达比B2细胞高1.3倍。观察到的变化与肌酸激酶活性或肌红蛋白的变化无显著相关。综上所述,对偏心臂运动的适应与循环中性粒细胞减少以及中性粒细胞和单核细胞激活状态降低有关。
The primary purpose of the study was to examine circulating neutrophils and monocytes and their plasma membrane expression of CD64, CD11b, and CD18 after two bouts (B1 and B2) of eccentric exercise. Subjects (n = 10) performed 25 forced-lengthened contractions of the forearm flexors on two occasions separated by 3 wk. Blood samples were obtained before exercise and at 1.5, 6, 12, 24, 48, 72, and 96 h of recovery. CD64, CD11b, and CD18 expression was determined via direct immunofluorescence and used as an indicator of neutrophil and monocyte activation. Creatine kinase activity (B1 = 1,390, B2 = 108 U/l), myoglobin (B1 = 163, B2 = 41, ng/dl), and muscle soreness and tenderness were higher (P < 0.01) after B1 compared with B2. Neutrophils at 6, 12, and 96 h were higher (P < 0.05) for B1 vs. B2. CD11b expression on neutrophils was 2.7-fold higher at 72 h for B1 vs. B2. CD64 expression on neutrophils at 72 and 96 h was 1.4- and 1.9-fold higher, respectively, for B1 vs. B2. At 72 and 96 h, CD18 and CD64 expression on monocytes was 1.3-fold higher for B1 vs. B2. The observed changes were not significantly correlated with changes in creatine kinase activity or myoglobin. In conclusion, the adaptation to eccentric arm exercise was associated with a reduction in circulating neutrophils and a lower state of neutrophil and monocyte activation.
免疫干扰素增强人粒细胞的功能特性:Fc 受体的作用以及淋巴毒素、肿瘤坏死因子和粒细胞-巨噬细胞集落刺激因子的作用。
DOI: --
发表时间: 1987
期刊: Journal of immunology (Baltimore, Md. : 1950)
影响因子: --
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DOI: --
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DOI: --
发表时间: 1987
期刊: Journal of immunology (Baltimore, Md. : 1950)
影响因子: --
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