The sensitizing capacity of Alstroemeria cultivars in man and guinea pig
The sensitizing capacity of Alstroemeria cultivars in man and guinea pig
复制标题
六出花品种对人和豚鼠的致敏能力
DOI：
10.1111/j.1600-0536.1983.tb04625.x
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发表时间：
1983
影响因子：
5.5
通讯作者：
H. Schubert
中科院分区：
文献类型：
--
作者：
B. Hausen;E. Prater;H. Schubert
在8名苗圃女工处理Alstroemeria ligtu品种的皮肤病中，有6例被证明是过敏性的。用切花提取物以及用分离和纯化的致敏剂进行的表皮试验均为阳性。成功的动物实验证实了Alstroemeria品种的致敏能力。负责但不稳定的接触性过敏原。α-亚甲基-γ-丁内酯（tulipalin A）在花瓣的短醚提取物中发现，浓度高达18%。由于其不稳定性，随后用甲醇进行提取，纯化后得到糖苷形式的致敏成分（郁金香苷A）。这可以在室温下储存更长时间而不损失活性。在14个不同来源的各种颜色的Alstroemeria ligtu品种的标本中测定了郁金香甙A：其含量在1和2%之间变化。在人类患者中直接测试植物材料具有假阳性反应和主动致敏的风险，因为这两种形式的过敏原的阈值非常高。只有0.01%的浓度可以被认为是安全的。
Dermatitis in 8 female nursery workers handling Alstroemeria ligtu cultivars has been proven in 6 cases to be of allergic origin. Epicutaneous tests with cut flower extracts as well as with the isolated and purified sensitizer were positive. Successful animal experiments corroborated the sensitising capacity of Alstroemeria cultivars. The responsible but unstable contact allergen. α‐methylene‐γ‐butyrolactone (tulipalin A), was found in short ether extracts of flower petals in concentrations up to 18%. Due to its instability, subsequent extractions were performed with methanol, yielding the sensitising constituent after purification in the glucosidic form (tuliposide A). This could be stored at room temperature for longer periods without loss of activity. Tuliposide A was determined in specimens of Alstroemeria ligtu cultivars of 14 different origins of various colours: its content varied between 1 and 2%. Direct testing of the plant material in human patients carries the risk of false positive reactions and active sensitization, as the threshold for both forms of the allergen is very high. Only a concentration of 0.01% can be considered safe.