Use of hydroxyethyl starch for inducing red blood cell aggregation
Use of hydroxyethyl starch for inducing red blood cell aggregation
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DOI：
10.3233/ch-2012-1542
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发表时间：
2012-01-01
影响因子：
2.1
通讯作者：
Busscher, Henk J.
中科院分区：
文献类型：
--
作者：
Henkelman, Sandra;Rakhorst, Gerhard;Busscher, Henk J.
人类红细胞（RBC）的聚集仍然具有生物学和临床意义。用聚合物替代血浆蛋白诱导红细胞聚集可能有助于揭示聚集过程的基本原理。存在两种理论来解释红细胞聚集机制：耗尽理论和桥接理论。羟乙基淀粉（HES）诱导的红细胞聚集随着聚合物尺寸的增加而增加，这表明聚集是通过桥接理论诱导的。在本研究中，电泳迁移率（EPM）的测定研究了200 kDa HES聚合物诱导红细胞聚集。此外，我们评估了这些聚合物是否有助于证明健康和1型糖尿病（T1DM）受试者的红细胞聚集差异。我们的研究结果表明，红细胞在200 kDa HES溶液中的EPM值小于悬浮液粘度预测的负值，支持桥接理论。此外，使用LORCA的聚合分析表明，200 kDa的HES溶液与标准500 kDa的葡聚糖溶液相似，增强了健康和糖尿病受试者的红细胞聚集。总之，我们的数据支持200 kDa HES诱导红细胞聚集的桥接机制。此外，这两种聚合物都可用于证明健康和T1DM受试者红细胞细胞诱导聚集的差异。
Aggregation of human red blood cells (RBC) remains of biological and clinical interest. Replacement of plasma proteins by polymers to induce RBC aggregation may help to unravel the fundamentals of the aggregation process. Two theories exist to explain RBC aggregation mechanisms: a depletion and a bridging theory. RBC aggregation induced by hydroxyethyl starch (HES) increases with polymer size, which suggests that aggregation is induced via the bridging theory. In this study, the electrophoretic mobility (EPM) was measured to investigate RBC aggregation induced by 200 kDa HES polymers. Also, we evaluated if these polymers were useful for demonstrating aggregation differences between RBCs from healthy and type-1 diabetes mellitus (T1DM) subjects. Our results demonstrate that EPM values of RBCs in 200 kDa HES solutions were less negative than could be predicted by the viscosity of the suspension, supporting the bridging theory. Furthermore, aggregation analysis using the LORCA demonstrated that 200 kDa HES solution enhanced RBC aggregation of healthy and diabetic subjects similarly as standard 500 kDa dextran solutions. In conclusion: our data supports the bridging mechanism underlying 200 kDa HES induced RBC aggregation. In addition, both polymers are useful for demonstrating cellular induced aggregation differences between RBCs from healthy and T1DM subjects.