Quantification of drug-induced fractional killing using high-throughput microscopy.
Quantification of drug-induced fractional killing using high-throughput microscopy.
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DOI：
10.1016/j.xpro.2021.100300
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Dixon SJ
中科院分区：
文献类型：
--
作者：
Inde Z;Rodencal J;Dixon SJ
抗癌药物在任何给定时间都只能杀死群体中的一小部分细胞。在这里，我们描述了一个协议，随着时间的推移，使用高通量成像定量药物诱导的部分杀伤。该协议可用于并行比较数百种条件的影响。我们展示了如何使用该协议来检查响应于有丝分裂原活化蛋白激酶途径抑制剂的分数杀伤。有关本方案使用和执行的完整详细信息，请参阅。体外药物诱导的部分杀伤的高通量分析方案比较不同条件下的部分杀伤的详细方法MEK 1/2介导的部分杀伤变异性的描述抗癌药物在任何给定的时间只杀死一部分细胞。在这里，我们描述了一个协议，随着时间的推移，使用高通量成像定量药物诱导的部分杀伤。该协议可用于并行比较数百种条件的效果。我们展示了如何使用该协议来检查响应于有丝分裂原活化蛋白激酶途径抑制剂的分数杀伤。
Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging. This protocol can be used to compare the effect of hundreds of conditions in parallel. We show how this protocol can be used to examine fractional killing in response to inhibitors of the mitogen-activated protein kinase pathway. For complete details on the use and execution of this protocol, please refer to. Protocol for high-throughput analysis of drug-induced fractional killing in vitro Detailed methods for comparing fractional killing between conditions Description of MEK1/2 inhibitor-induced fractional killing variability Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging. This protocol can be used to compare the effect of hundreds of conditions in parallel. We show how this protocol can be used to examine fractional killing in response to inhibitors of the mitogen activation protein kinase pathway.
影响因子：
9.3
作者：
Forcina GC;Conlon M;Wells A;Cao JY;Dixon SJ
通讯作者：
Dixon SJ
影响因子：
8.8
作者：
Inde, Zintis;Forcina, Giovanni C.;Dixon, Scott J.
通讯作者：
Dixon, Scott J.