Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species
Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species
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DOI：
10.1007/bf00417944
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发表时间：
1996-09-01
影响因子：
5.4
通讯作者：
McFerson, JR
中科院分区：
文献类型：
--
作者：
SzewcMcFadden, AK;Kresovich, S;McFerson, JR
简单重复序列（SSR）基因分型技术在作物遗传变异分析中的应用受到了限制，因为SSR引物的易得性和重复序列在相关物种中的保守性有限。在这一阶段的工作中，我们报告的鉴定和表征的SSR是保守的甘蓝型油菜L。(rape种子）及其推定的祖细胞，B。oleracea L.（卷心菜和相关蔬菜类型）和B。芜菁（蔬菜和油类）。从大小分级的B基因组文库中获得约140个克隆。对21个二核苷酸SSR引物进行了设计。17对引物在3种植物中扩增出了特异性条带。其中13个发现了物种之间和物种内的变异。与人类SSR信息含量的研究结果不同，靶序列内串联重复序列的数量与杂合性之间没有关系。所有引物对都被设计成在相同的扩增条件下工作：因此：使用这些SSR的单反应、多重聚合酶链反应（PCR）是可能的。一旦用户社区可以获得中等数量的引物对，SSR基因分型可能为农作物多样性的表征、保护和利用提供有用的方法。
The application of simple sequence repeat (SSR) genotyping for the characterization of genetic variation in crop plants has been hindered bq ready access to useful primer pairs and potentially limited conservation of the repeat sequences among related species. In this phase of work, we report on the identification and characterization of SSRs that are conserved in Brassica napus L. (rape seed) and its putative progenitors, B. oleracea L. (cabbage, and related vegetable types) and B. rapa (vegetable and oil types). Approximately 140 clones from a size-fractionated genomic library of B. napus were sequenced, and primer pairs were designed for 21 dinucleotide SSRs. Seventeen primer pairs amplified products in the three species and. among these, 13 detected variation between and within species. Unlike findings on SSR information content in human no relationship could be established between the number of tandem repeats within the target sequence and heterozygosity. All primer pairs have been designed to work under identical amplification conditions: therefore: single-reaction, multiplex polymerase chain reaction (PCR) with these SSRs is possible. Once moderate numbers of primer pairs are accessible to the user community, SSR genotyping may provide a useful method for the characterization, conservation, and utilization of agricultural crop diversity.