Exosomes from gingival mesenchymal stem cells enhance migration and osteogenic differentiation of pre-osteoblasts
Exosomes from gingival mesenchymal stem cells enhance migration and osteogenic differentiation of pre-osteoblasts
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DOI：
10.1691/ph.2020.0652
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发表时间：
2020-11-01
期刊：
影响因子：
1.6
通讯作者：
Xu, Liqin
中科院分区：
文献类型：
--
作者：
Jiang, Shengjun;Xu, Liqin
牙龈间充质干细胞在骨组织再生方面具有巨大的潜力。然而，目前尚不清楚来源于GMSC的外泌体如何影响骨相关细胞的功能。在这项研究中，我们探讨了GMSCs-derived exosomes（GMSCs-Exos）对前成骨细胞MC 3 T3-E1增殖，迁移和成骨分化的影响。CCK-8检测结果显示GMSCs-Exos对前成骨细胞的增殖无影响。此外，我们发现GMSCs-Exos促进前成骨细胞的迁移和MC 3 T3-E1的成骨分化，如通过增强茜素红染色、升高碱性磷酸酶（ALP）活性和上调成骨基因的表达所揭示的。本研究为GMSCs在骨再生中外泌体介导的旁分泌机制提供了新的见解。
Gingival mesenchymal stem cells (GMSCs) have great potential in bone tissue regeneration. However, it is not well known how on exosomes derived from GMSCs affect the functions of bone-related cells. In this study, we explored the impact of GMSCs-derived exosomes (GMSCs-Exos) on pre-osteoblast MC3T3-E1 proliferation, migration and osteogenic differentiation. Results of CCK-8 assay showed that GMSCs-Exos had no effect on proliferation of pre-osteoblasts. Further, we found that GMSCs-Exos promoted the migration of pre-osteoblasts and osteogenic differentiation of MC3T3-E1 as revealed by enhanced Alizarin red staining, elevated alkaline phosphatase (ALP) activity and upregulated expression of osteogenic genes. This study provides new insights into the potential exosome-mediated paracrine mechanism of GMSCs in bone regeneration.