Antidepressant effects of the water extract from Taraxacum officinale leaves and roots in mice
Antidepressant effects of the water extract from Taraxacum officinale leaves and roots in mice
复制标题
蒲公英叶和根水提取物对小鼠的抗抑郁作用
DOI：
10.3109/13880209.2013.876432
复制
发表时间：
2014-08-01
影响因子：
3.8
通讯作者：
Huang, Qi
中科院分区：
文献类型：
--
作者：
Li, Yu-Cheng;Shen, Ji-Duo;Huang, Qi
摘要内容：蒲公英（Taraxacum officinale F.）（菊科）是东亚国家广泛使用的传统草药。目的：研究雷公藤水提物的抗抑郁作用。采用小鼠强迫游泳试验（FST）、悬尾试验（TST）和旷场试验（OFT），研究了铁皮石斛（WETO）叶和根对小鼠的抗氧化作用。材料和方法：急性（1天）和慢性治疗（14天）与WETO（50，100和200 mg/kg）对FST，TST和OFT的行为变化，以及血清促肾上腺皮质激素释放因子（CRF），促肾上腺皮质激素（ACTH）和皮质酮浓度的影响进行了评估。结果如下：WETO以50、100和200 mg/kg剂量长期给药（14天）可显著缩短FST（92.6、85.1和77.4 s）和TST（84.8、72.1和56.9 s）的不动时间。WETO以200 mg/kg的剂量进行急性处理（1天）也显著减少了FST（81.7 s）和TST（73.2 s）的不动时间。然而，所有的治疗没有影响的运动活动的OFT。此外，FST诱导血清CRF（5.8 ng/ml）、ACTH（104.7 pg/ml）和皮质酮（37.3 ng/ml）水平显著升高。WETO长期给药（14天）可降低血清CRF（200 mg/kg：3.9 ng/ml）和皮质酮（50 mg/kg：29.9 ng/ml; 100 mg/kg：22.5 ng/ml; 200 mg/kg：19.8 ng/ml）水平。讨论与结论：这些结果清楚地证明了WETO在行为绝望动物模型中的抗抑郁作用，并提示了神经内分泌系统参与的机制。
Abstract Context: The leaves and roots of the Taraxacum officinale F. (Asteraceae) is widely used as traditional medicinal herb in Eastern Asian countries. Objective: In the present study, the antidepressant-like effects of the water extract of T. officinale (WETO) leaves and roots were investigated in mice using forced swimming test (FST), tail suspension test (TST) and open field test (OFT). Materials and methods: Effects of acute (1-day) and chronic treatments (14-days) with WETO (50, 100 and 200 mg/kg) on the behavioral changes in FST, TST and OFT, and the serum corticotrophin releasing factor (CRF), adrenocorticotropic hormone (ACTH) and corticosterone concentration were assessed in mice. Results: Chronic treatment (14-days) with WETO at the doses of 50, 100 and 200 mg/kg significantly decreased the immobility time in both FST (92.6, 85.1 and 77.4 s) and TST (84.8, 72.1 and 56.9 s). Acute treatment (1-day) with WETO at a dose of 200 mg/kg also markedly decreased the immobility time in both FST (81.7 s) and TST (73.2 s). However, all treatments did not affect the locomotor activity in the OFT. Moreover, FST induced a significant increase in serum CRF (5.8 ng/ml), ACTH (104.7 pg/ml) and corticosterone levels (37.3 ng/ml). Chronic treatment (14-days) with WETO decreased the serum CRF (200 mg/kg: 3.9 ng/ml) and corticosterone (50 mg/kg: 29.9 ng/ml; 100 mg/kg: 22.5 ng/ml; 200 mg/kg: 19.8 ng/ml) levels. Discussion and conclusion: These results clearly demonstrated the antidepressant effects of WETO in animal models of behavioral despair and suggested the mechanism involved in the neuroendocrine system.