The preventive effect of a free radical scavenger on oxidative stress after the relief of partial bladder outlet obstruction in a rat model
The preventive effect of a free radical scavenger on oxidative stress after the relief of partial bladder outlet obstruction in a rat model
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DOI：
10.1371/journal.pone.0199800
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发表时间：
2018-10-22
期刊：
影响因子：
3.7
通讯作者：
Oh, Seung-June
中科院分区：
文献类型：
--
作者：
Choo, Min Soo;Piao, SongZhe;Oh, Seung-June
目的探讨自由基清除剂tempol对部分膀胱出口梗阻（pBOO）大鼠膀胱功能的影响。将大鼠随机分为5组：假手术组、Tempo处理1周组（Treat-1 w）、Tempo处理3周组（Treat-3 w）、Tempo不处理1周组（non-Treat-1 w）、Tempo不处理3周组（non-Treat-3 w）。根据分组，在缓解后1周或3周进行清醒膀胱测压。分离膀胱并称重。HE染色、Masson三色染色、TUNEL染色观察组织学变化。氧化应激采用丙二醛法。结果Tempo处理组每一排尿周期的非排尿性收缩次数显著减少，与对照组比较，差异有统计学意义（P <0.05）（非治疗-1周vs.治疗-1周，1.18 +/- 0.82 vs. 0.36 +/- 0.40，P = 0.010;非治疗-3周vs.治疗-3周，1.51 +/- 0.69 vs. 0.23 +/- 0.25，P = 0.002）。治疗组逼尿肌层厚度和胶原纤维沉积明显减少。检测到的细胞凋亡主要在尿路上皮细胞层中观察到，尽管给药组的细胞凋亡率显著降低（48.9 +/- 3.36% vs. 32.7 +/-11.10%，P = 0.024; 25.8 +/- 4.67% vs. 15.7 +/-9.83%，P = 0.314）。Tempo治疗组在缓解后1周和3周均显示MDA浓度显著降低。β 3肾上腺素受体的表达增加tempo治疗rats.ConclusionsIschemic再灌注损伤后缓解pBOO引起的膀胱组织学和功能的变化。自由基清除剂治疗防止这种氧化应激。
AimsTo investigate the effect of a free radical scavenger (tempol) after relief of partial bladder outlet obstruction (pBOO) on bladder function in a rat model.MethodspBOO was induced in 50 eight-week-old female Sprague-Dawley rats and relieved 3 weeks later. The rats were divided randomly into 5 groups: sham-operated, tempol-treated for 1 week (Treat-1w) or 3 weeks (Treat-3w), and no treatment for 1 week (nonTreat-1w) or 3 weeks (nonTreat-3w). Awaken cystometrograms were obtained 1 or 3 weeks after relief according to the grouping. The bladders were isolated and weighed. H&E, Masson's trichrome and TUNEL staining were used to analyze histological changes. The oxidative stress assessed using malondialdehyde. The expression of beta-3 adrenoreceptor was examined by Western blotting.ResultsThe tempol-treated groups exhibited a significant decrease in the number of non-voiding contractions per voiding cycle (nonTreat-1w vs. Treat-1w, 1.18 +/- 0.82 vs. 0.36 +/- 0.40, P = 0.010; nonTreat-3w vs. Treat-3w, 1.51 +/- 0.69 vs. 0.23 +/- 0.25, P = 0.002). The thickness and collagen fiber deposition of the detrusor muscle layer was significantly decreased in the treated groups. Apoptosis detected was mainly observed in the urothelial cell layer, although the rate of apoptosis was significantly decreased in the treated groups (48.9 +/- 3.36% vs. 32.7 +/- 11.10%, P = 0.024; 25.8 +/- 4.67% vs. 15.7 +/- 9.83%, P = 0.314). The tempol-treated groups showed significant decreases in the MDA concentrations at both 1 and 3 weeks after relief. The expression of the beta-3 adrenoreceptor was increased in the tempol-treated rats.ConclusionsIschemic reperfusion injury after relief of pBOO caused histological and functional changes in the bladder. Free radical scavenger treatment prevented this oxidative stress.