Prostaglandin E2 receptor subtypes, EP1, EP2, EP3 and EP4 in human and mouse ocular tissues -: a comparative immunohistochemical study
Prostaglandin E2 receptor subtypes, EP1, EP2, EP3 and EP4 in human and mouse ocular tissues -: a comparative immunohistochemical study
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DOI：
10.1016/j.plefa.2004.03.021
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发表时间：
2004-11-01
影响因子：
3
通讯作者：
Paterson, CA
中科院分区：
文献类型：
--
作者：
Biswas, S;Bhattacherjee, P;Paterson, CA
本研究的目的是比较前列腺素E-2受体亚型在正常人和小鼠眼组织中的定位。用EP1、EP2、EP3和EP4特异性抗体处理正常人和小鼠(129Sv/ev)眼的石蜡切片，然后与Alexa Fluor二抗(Ex/Em=555/571)孵育，检测EP受体蛋白的存在。定位抗体的荧光在Carl Zeiss显微镜(Axiovert 200)中可视化，并用Carl Zeiss Axiocam相机拍摄。在小鼠中，EP和EP3受体亚型仅适度表达，EP3受体表达几乎可以忽略不计。在人的角膜和虹膜睫状体中，EP1和EP3受体均有显著表达。EP4受体在人和小鼠眼组织中呈中等水平表达。EP2受体是人和小鼠眼组织中表达最显著和最丰富的受体。结论：人和小鼠眼组织中EP受体亚型的分布规律相似。因此，129株Sv/Ev小鼠可作为研究前列腺素受体激动剂眼部病理生理作用的合适动物模型。(C)2004爱思唯尔有限公司。保留所有权利。
The purpose of the present study was to compare the localization of prostaglandin E-2 receptor subtypes in normal human and mouse ocular tissues. Paraffin embedded sections of normal human and mouse (129 Sv/Ev) eyes were treated with EP1, EP2, EP3 and EP4 specific antibodies and subsequently incubated with Alexa Fluor secondary antibody (Ex/Em = 555/571) to detect the presence of EP receptor proteins. Fluorescence of the localized antibodies was visualized in a Carl Zeiss Microscope (Axiovert 200) and photographed using Carl Zeiss Axiocam camera. In mice EP, and EP3 receptor subtypes were only moderately expressed, EP3 receptor expression being almost negligible. In human cornea and iris ciliary body, EP1 and EP3 receptors were prominently expressed. EP4 receptor was expressed moderately in human and mouse ocular tissues. EP2 receptor was the most prominently and abundantly expressed receptor in both human and mouse ocular tissues. It is concluded that the pattern of the distribution of EP receptor subtypes in the ocular tissues are similar in human and mouse. Thus, 129 Sv/Ev strains of mice would make an appropriate animal model for studying the ocular pathophysiological roles of prostaglandin receptor agonists. (C) 2004 Elsevier Ltd. All rights reserved.