Antibacterial activity of chitosan nanofiber meshes with liposomes immobilized releasing gentamicin
Antibacterial activity of chitosan nanofiber meshes with liposomes immobilized releasing gentamicin
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DOI：
10.1016/j.actbio.2015.02.018
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发表时间：
2015-05-01
影响因子：
9.7
通讯作者：
Neves, Nuno M.
中科院分区：
文献类型：
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作者：
Monteiro, Nelson;Martins, Margarida;Neves, Nuno M.
Chitsan (Ch) 纳米纤维网 (NFM) 是一种具有自然特性的材料，有利于其在人体伤口敷料中的应用。目前的工作提出了一种负载庆大霉素的脂质体固定在 Ch NFM 表面以提高其抗菌活性。为了实现这一目的，Ch NFMs 用硫醇基团进行功能化，并通过 SH 基团与马来酰亚胺的反应共价固定负载庆大霉素的脂质体。在pH 7 时获得SH 基团的最大浓度(55.52 +/- 11.19 nmol cm(-2))。荧光染料与电纺Ch NFM 表面的SH 基团共价结合。当通过荧光显微镜分析时，它们的空间分布在整个 NFM 中是均匀的。庆大霉素被成功封装到脂质体中，效率为17%。负载庆大霉素的脂质体均匀分布在 Ch NFM 的表面，药物释放动力学显示庆大霉素在 16 小时内持续释放，并在 24 小时达到稳定状态。体外药敏试验证实，电纺Ch NFM表面固定的脂质体释放的庆大霉素对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌具有杀菌活性。结果表明，所开发的系统在伤口敷料应用中具有良好的性能，可避免这些常见病原体引起的感染。 (C) 2015 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Chitsan (Ch) nanofiber mesh (NFM) is a material with natural characteristics favoring its use in human wound dressing. The present work proposes a gentamicin-loaded liposome immobilized at the surface of Ch NFMs to promote its antibacterial activity. To achieve this purpose, Ch NFMs were functionalized with thiol groups, and gentamicin-loaded liposomes were covalently immobilized by the reaction of the SH groups with maleimide. The maximum concentration of SH groups (55.52 +/- 11.19 nmol cm(-2)) was obtained at pH 7. A fluorescent dye was covalently bound to the SH groups present at the surface of electrospun Ch NFMs. Their spatial distribution was uniform throughout the NFMs when analyzed by fluorescence microscopy. Gentamicin was successfully encapsulated into the liposomes with an efficiency of 17%. Gentamicin-loaded liposomes were uniformly distributed at the surface of the Ch NFMs and the drug release kinetic showed a sustained release of gentamicin during 16 h, achieving a steady state at 24 h. The in vitro susceptibility tests confirmed that the gentamicin released from the liposomes immobilized at the surface of electrospun Ch NFM has bactericidal activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The results show that the developed system has promising performance for wound dressing applications, avoiding infections caused by these common pathogens. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.