MOLECULAR-CLONING, TISSUE EXPRESSION OF HUMAN XANTHINE DEHYDROGENASE
MOLECULAR-CLONING, TISSUE EXPRESSION OF HUMAN XANTHINE DEHYDROGENASE
复制标题
DOI：
10.1006/bbrc.1994.1328
复制
发表时间：
1994-03-15
影响因子：
3.1
通讯作者：
HOIDAL, JR
中科院分区：
文献类型：
--
作者：
XU, P;HUECKSTEADT, TP;HOIDAL, JR
黄嘌呤脱氢酶（XDH，EC 1.1.1.204）是氧化多种嘌呤、蝶呤和其它异源氮化合物的钼铁-硫黄素羟化酶，在核酸降解中充当限速酶。在这项工作中，我们已经分离和测序人肝XDH的cDNA克隆。所获得的cDNA全长4577 bp，5 '端非翻译区63 bp，3'端非翻译区515 bp。鉴定了终止密码子TGA和多聚腺苷酸化信号AATAAA。开放阅读框编码1333个氨基酸残基。通过直接测序纯化人乳XDH的该区域来确认N-末端的分配。北方印迹分析表明，人XDH基因在人体组织中广泛表达。(C)1994年出版社出版。
Xanthine dehydrogenase (XDH, EC 1.1.1.204) is a molybdenum iron-sulphur flavin hydroxylase which oxidizes a variety of purines, pterins and other heterogenic nitrogen compounds, serving as a rate-limiting enzyme in nucleic acid degradation. In this work, we have isolated and sequenced cDNA clones of human liver XDH. The obtained cDNA covers 4577 bases of human liver XDH mRNA with a 63 bp 5'-end untranslated region and a 515 bp 3'-end untranslated region. A termination codon TGA and a polyadenylation signal AATAAA were identified. An open reading frame encodes 1333 amino acid residues. The assignment of the N-terminal was confirmed by directly sequencing that region of purified human milk XDH. Northern blot analysis shows that the human XDH gene is widely expressed in human tissues. (C) 1994 Academic Press, Inc.