Comparison of blood-brain barrier permeability in mice and rats using in situ brain perfusion technique
Comparison of blood-brain barrier permeability in mice and rats using in situ brain perfusion technique
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DOI：
10.1152/ajpheart.2000.279.3.h1022
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发表时间：
2000-09-01
影响因子：
4.8
通讯作者：
Sawada, Y
中科院分区：
文献类型：
--
作者：
Murakami, H;Takanaga, H;Sawada, Y
在这里，我们提出了一种测量小鼠血脑屏障处的渗透系数-表面积乘积（PS）值的方法，该方法使用最初由Takasato等人（Am J Physiol Heart Circ Physiol 247：H484-H493，1984）为大鼠开发的原位脑灌注技术。逆行注入右颈外动脉增加颈动脉灌注压的比例灌注率。在1.0 ml/min的灌注速率下，小鼠的血管内容积和脑灌注液流量与大鼠相似。此外，体循环血液对半球总流量的贡献很小（仅3.2%）。这些发现表明这种灌注速率适合于小鼠。用原位脑灌注技术测定了20多种不同化合物的PS值，并与大鼠的数据进行了比较。小鼠和大鼠的PS值之间存在密切的关系（1：1），表明小鼠和大鼠的脑毛细血管通透性相似。
Here we present a method for measuring the permeability coefficient-surface area product (PS) values at the blood-brain barrier in mice, using the in situ brain perfusion technique originally developed for rats by Takasato et al. (Am J Physiol Heart Circ Physiol 247: H484-H493, 1984). Retrograde infusion into the right external carotid artery increased the carotid perfusion pressure in proportion to the perfusion rate. Intravascular volume and cerebral perfusion fluid flow at a perfusion rate of 1.0 ml/min in mice were similar to those in rats. In addition, the contribution of systemic blood to total flow in the hemisphere was small (only 3.2%). These findings indicated that this perfusion rate is suitable for mice. The PS values of more than 20 different compounds were determined in mice by using the in situ brain perfusion technique, and comparisons were made with data from rats. There was a close relationship (1:1) between the PS values in mice and rats, indicating that brain capillary permeabilities are similar in mice and rats.