Design of a new electrospray ion mobility mass spectrometer
Design of a new electrospray ion mobility mass spectrometer
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DOI：
10.1016/s1387-3806(01)00517-6
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发表时间：
2001-12-20
影响因子：
1.8
通讯作者：
Bowers, MT
中科院分区：
文献类型：
--
作者：
Wyttenbach, T;Kemper, PR;Bowers, MT
提出了一种新型离子淌度质谱仪的设计。该设计采用电喷雾离子源；离子漏斗，用于将离子有效地从源传输到迁移池并以脉冲离子模式积累离子；流动池和四极质量分析器。仪器的每个部分都有详细的描述。使用新仪器获得的初步结果展示了其功能。平衡实验表明，向双质子化肽缓激肽、血管紧张素 II 和 LHRH 添加第一个水分子的 DeltaG 度 (300 K) 值在 -3.5 至 -2.5 kcal/mol 范围内。对于血管紧张素 II 和 LHRH，单质子化离子的相应值 > -0.5 kcal/mol，但对于缓激肽则等于 -2.6 kcal/mol。缓激肽中更强的结合可能是由于盐桥结构的存在。离子到达时间分布表明，单质子化肽可以形成 (nM + nH)(n+) 形式的聚集体。这些离子的迁移率表明它们接近球形。将漂移池加热至大约 450 K 会导致 (2M + 2H)(2-) 离子在 1 ms 实验时间尺度上解离为两个 (M + H)(+) 单元。与实验数据的理论拟合得出了缓激肽的速率常数和解离屏障为 30 +/- 2 kcal/mol，LHRH 的解离屏障为 39 +/- 3 kcal/mol。 (C) 2001 Elsevier Science B.V.
The design of a new ion mobility mass spectrometer is presented. The design features an electrospray ion source; an ion funnel to transmit ions efficiently from the source to the mobility cell and to accumulate ions in the pulsed ion mode; a mobility cell, and a quadrupole mass analyzer. Each part of the instrument is described in detail. Preliminary results obtained with the new instrument are presented to demonstrate its capabilities. Equilibrium experiments showed that the DeltaG degrees (300 K) values for the addition of the first water molecule to the doubly protonated peptides bradykinin, angiotensin II, and LHRH are in the range from -3.5 to -2.5 kcal/mol. The corresponding values for the singly protonated ions are > -0.5 kcal/mol for angiotensin II and LHRH, but equal to -2.6 kcal/mol for bradykinin. The stronger bonding in bradykinin may be due to the presence of a salt bridge structure. Ion arrival time distributions showed that singly protonated peptides can form aggregates of the form (nM + nH)(n+). The mobilities of these ions indicated that they are near spherical. Heating the drift cell to similar to 450 K caused dissociation of the (2M + 2H)(2-) ion into two (M + H)(+) units on the 1 ms experimental time scale. A theoretical fit to the experimental data yielded rate constants and a barrier for dissociation of 30 +/- 2 kcal/mol for bradykinin and 39 +/- 3 kcal/mol for LHRH. (C) 2001 Elsevier Science B.V.