A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes.
A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes.
复制标题
DOI：
10.1093/jxb/erv470
复制
发表时间：
2016-02
影响因子：
6.9
通讯作者：
Price AH
中科院分区：
文献类型：
--
作者：
Dimkpa SO;Lahari Z;Shrestha R;Douglas A;Gheysen G;Price AH
通过对331个水稻品种的抗性鉴定，鉴定出2个抗性地方品种、11个数量性状基因座和良好的抗禾本科根结线虫候选基因。禾谷根结线虫是世界范围内危害最严重的线虫之一，严重制约着水稻生产。虽然亚洲水稻对白粉病的抗性存在变异，但到目前为止，还没有强而可靠的抗性报道。已经报道了部分抗性的数量性状基因座，但还没有标记或克隆潜在基因。在这里，水稻多样性小组1的332份材料被评估了鳞癌的形成，揭示了水稻所有亚群的巨大变异和在温带粳稻材料中更高的感受性。Kao Pahk Maw和LD 24表现为抗性，这一点在大型盆栽试验中得到了证实，没有观察到虫害。对这两份材料的详细观察表明，接种2天后，根内没有线虫，17天后几乎没有雌性线虫(Kao Pahk Maw有5条，LD 24有1条，而感病对照为>；100)。这两个品种是抗根结线虫育种的理想供体。全基因组关联研究揭示了11个数量性状基因座，其中两个与在Bala x Azucena种群中检测到的上位性基因座相近。讨论了一些值得进一步探索的候选基因，特别是许多具有凝集素结构域的基因和位于11号染色体上与大麦MLA基因同源的基因。
Assessing gall numbers in 331 cultivars of a rice diversity panel has identified two resistant landraces, 11 quantitative trait loci and good candidate genes for resistance to Meloidogyne graminicola. The root-knot nematode Meloidogyne graminicola is one of the most serious nematode pests worldwide and represents a major constraint on rice production. While variation in the susceptibility of Asian rice (Oryza sativa) exists, so far no strong and reliable resistance has been reported. Quantitative trait loci for partial resistance have been reported but no underlying genes have been tagged or cloned. Here, 332 accessions of the Rice Diversity Panel 1 were assessed for gall formation, revealing large variation across all subpopulations of rice and higher susceptibility in temperate japonica accessions. Accessions Khao Pahk Maw and LD 24 appeared to be resistant, which was confirmed in large pot experiments where no galls were observed. Detailed observations on these two accessions revealed no nematodes inside the roots 2 days after inoculation and very few females after 17 days (5 in Khao Pahk Maw and <1 in LD 24, in comparison with >100 in the susceptible controls). These two cultivars appear ideal donors for breeding root-knot nematode resistance. A genome-wide association study revealed 11 quantitative trait loci, two of which are close to epistatic loci detected in the Bala x Azucena population. The discussion highlights a small number of candidate genes worth exploring further, in particular many genes with lectin domains and genes on chromosome 11 with homology to the Hordeum Mla locus.