Characterization of sperm protein 17 in human somatic and neoplastic tissue
Characterization of sperm protein 17 in human somatic and neoplastic tissue
复制标题
DOI：
10.1016/s0304-3835(02)00350-6
复制
发表时间：
2002-12-05
期刊：
影响因子：
9.7
通讯作者：
Robbins, D
中科院分区：
文献类型：
--
作者：
De Jong, A;Buchli, R;Robbins, D
精子蛋白17（Sp17）是一种高度抗原性的睾丸特异性蛋白，其已知功能是将精子结合到透明精子上。然而，Sp17基因最近已被检测到在正常的非睾丸组织和恶性肿瘤。由于Sp17在非睾丸组织中的作用尚不清楚，因此在高度增殖组织中Sp17基因的表征可能为进一步了解Sp17的调节和替代功能提供了依据。同样，通过PCR检测Sp17-2基因。采用北方和Western blot方法检测Sp17 mRNA和蛋白的表达。Sp17-1a和Sp17-1b转录本从癌细胞系扩增。类似地，在癌细胞系中也检测到Sp17-2转录物。此外，北方印迹分析显示，Sp17 mRNA的表达在所有的癌细胞系检查。但未检测到Sp17蛋白表达。与正常非睾丸组织相比，癌细胞系中Sp17转录本的差异检测表明Sp17在病变细胞中具有潜在的致病作用。此外，Sp17-2转录物可能是高度增殖细胞的标志物。总的来说，这些数据暗示Sp17是癌症睾丸抗原。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Sperm protein 17 (Sp17) is a highly antigenic, testes-specific protein whose known function is to bind sperm to the zona pellucida. However, the Sp17 gene has been recently detected in normal non-testes tissues and malignant neoplasias. As the role of Sp17 in non-testes tissues is unknown, the characterization of the Sp17 gene in highly proliferating tissues may provide further insight into the regulation and alternative function of Sp17 Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify the Sp17-1 transcript in multiple normal human tissues and cancer cell lines. Similarly, the Sp17-2 gene was examined by PCR. In addition, Northern and Western blot analyses were used to detect Sp17 mRNA and protein expression. The Sp17-1a and Sp17-1b transcripts were amplified from cancer cell lines. Similarly, an Sp17-2 transcript was also detected in cancer cell lines. Furthermore, Northern blot analysis revealed Sp17 mRNA expression in all cancer cell lines examined. However, Sp17 protein expression was not detected. The differential detection of the Sp17 transcripts in cancer cell lines as compared to normal non-testes tissues, suggests a potential pathogenic role for Sp17 in diseased cells. Moreover, the Sp17-2 transcript may be a marker for highly proliferating cells. Collectively, these data implicate Sp17 as a cancer testis antigen. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.