Distinct Roles of Multiple NDH-1 Complexes in the Cyanobacterial Electron Transport Network as Revealed by Kinetic Analysis of P700+ Reduction in Various ndh-Deficient Mutants of Synechocystis sp. Strain PCC6803
Distinct Roles of Multiple NDH-1 Complexes in the Cyanobacterial Electron Transport Network as Revealed by Kinetic Analysis of P700+ Reduction in Various ndh-Deficient Mutants of Synechocystis sp. Strain PCC6803
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DOI：
10.1128/jb.00984-10
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Roegner, Matthias
中科院分区：
文献类型：
--
作者：
Bernat, Gabor;Appel, Jens;Roegner, Matthias
甲基紫精在KCN处理的野生型集胞藻PCC 6803和NdhF 3/NdhF 4（NdhF 3/F4）缺陷突变体中对远红脉冲后P700（+）再还原动力学的影响很小，而在NdhF 1缺陷突变体中，甲基紫精对P700（+）再还原的影响相当缓慢。这强烈表明，（i）从代谢产物到质体醌的活性电子流在ndhF 1缺失后受到抑制，（ii）光系统1介导的循环电子传递依赖于NdhF 3/F4型NDH-1复合物。
While methyl viologen had only a small effect on P700(+) rereduction kinetics after far-red pulses in KCN-treated wild-type Synechocystis sp. strain PCC6803 and an NdhF3/NdhF4 (NdhF3/F4)-defective mutant, it involved a rather slow P700(+) rereduction in an NdhF1-defective mutant. This strongly indicates that (i) active electron flow from metabolites to plastoquinone is suppressed upon deletion of ndhF1 and (ii) photosystem 1-mediated cyclic electron transport is dependent on NdhF3/F4-type NDH-1 complexes.