The transcriptional activity of human Chromosome 22
The transcriptional activity of human Chromosome 22
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DOI：
10.1101/gad.1055203
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发表时间：
2003-02-15
影响因子：
10.5
通讯作者：
Snyder, M
中科院分区：
文献类型：
--
作者：
Rinn, JL;Euskirchen, G;Snyder, M
构建了代表人类22号染色体几乎所有独特序列的DNA微阵列，并用于测量胎盘poly（A）（+）RNA的全局转录活性。我们发现许多已知的、相关的和预测的基因都得到了表达。更重要的是，我们的研究揭示了转录的碱基是以前报道的两倍。许多新发现的表达片段通过RNA印迹分析和一种称为差异杂交作图（DHM）的新技术进行了验证。有趣的是，这些新片段的显著部分与先前注释的内含子反义表达。这些新的表达区域的编码潜力是由它们在小鼠基因组中的序列保守性支持的。这项研究大大增加了我们对人类染色体上编码的生物信息的理解。为了便于将这些结果传播给科学界，我们开发了一个全面的网络资源，在http://array.mbb.yale.edu/chr22上展示这项研究的结果和人类22号染色体的其他特征。
A DNA microarray representing nearly all of the unique sequences of human Chromosome 22 was constructed and used to measure global-transcriptional activity in placental poly(A)(+) RNA. We found that many of the known, related and predicted genes are expressed. More importantly, our study reveals twice as many transcribed bases as have been reported previously. Many of the newly discovered expressed fragments were verified by RNA blot analysis and a novel technique called differential hybridization mapping (DHM). Interestingly, a significant fraction of these novel fragments are expressed antisense to previously annotated introns. The coding potential of these novel expressed regions is supported by their sequence conservation in the mouse genome. This study has greatly increased our understanding of the biological information encoded on a human chromosome. To facilitate the dissemination of these results to the scientific community, we have developed a comprehensive Web resource to present the findings of this study and other features of human Chromosome 22 at http://array.mbb.yale.edu/chr22.