Enamel structure properties controlled by engineered proteins in transgenic mice
Enamel structure properties controlled by engineered proteins in transgenic mice
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DOI：
10.1359/jbmr.2003.18.11.2052
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发表时间：
2003-11-01
影响因子：
6.2
通讯作者：
Sarikaya, M
中科院分区：
文献类型：
--
作者：
Fong, H;White, SN;Sarikaya, M
釉原蛋白对哺乳动物牙齿的釉质生物合成具有调节作用。使用从表达两种不同蛋白质工程版本的釉原蛋白的转基因小鼠获得的牙齿，我们进行了结构-纳米力学特性的关联，与年龄匹配的野生型小鼠相比，显示出21%的硬度和24%的弹性模数下降。我们将这些劣质特性归因于蛋白质基质的解体，导致矿物形成有缺陷。
Amelogenin protein has regulatory effects on enamel biofabrication in mammalian tooth. Using teeth obtained from transgenic mice that express two separate protein-engineered versions of amelogenins, we made structure-nanomechanical properties correlations and showed 21% hardness and 24% elastic modulus degradation compared with the age-matched wildtype littermates. We attribute the inferior properties to the disorganization of the protein matrix resulting in defective mineral formation.