Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy
Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy
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DOI：
10.1016/j.cellimm.2013.07.010
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发表时间：
2013-07-01
影响因子：
4.3
通讯作者：
Suzuki, Ryuji
中科院分区：
文献类型：
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作者：
Eguchi, Takanori;Kumagai, Kenichi;Suzuki, Ryuji
镍 (Ni) 可引起迟发型超敏反应，该反应被认为是由 T 细胞积聚到发炎皮肤中介导的。由于尚未建立合适的动物模型，金属过敏发育阶段积累的 T 细胞的特征尚不清楚。为了研究过敏性发炎皮肤中积累的 T 细胞，我们建立了一种新型镍诱导过敏的小鼠模型。通过将镍加脂多糖溶液进入腹股沟两次致敏，然后用镍溶液进入足垫进行三次刺激来诱导镍过敏的小鼠模型。在这里，我们展示了带有 V α 14J α 18 的特定 TCR 库，称为自然杀伤 (NK) T 细胞，在 BALB/c 或 C57BL/6 小鼠中单克隆扩增。 NKT细胞的积累被表征为CD4(+)或CD4(-)CD8(-)T细胞。这些结果表明NKT细胞是Ni过敏诱发阶段的主要致病性T细胞。 (C) 2013 Elsevier Inc. 保留所有权利。
Nickel (Ni) can cause delayed-type hypersensitivity reactions, which are thought to be mediated by the accumulation of T cells into inflamed skin. Accumulated T cells at the developmental stages in metal allergy are poorly characterized because a suitable animal model has not been established. To investigate the accumulated T cells in allergic inflamed skin, we generated a novel murine model of Ni-induced allergy. The murine model of Ni allergy was induced by two sensitizations of Ni plus lipopolysaccharide solution into the groin followed by three challenges with Ni solution into the footpad. Here we show that a specific TCR repertoire bearing V alpha 14J alpha 18, called natural killer (NK) T cells, was expanded monoclonally in BALB/c or C57BL/6 mice. Accumulation of NKT cells was characterized as CD4(+) or CD4(-)CD8(-) T cells. These results suggested that NKT cells are major pathogenic T cells at the elicitation phase of Ni allergy. (C) 2013 Elsevier Inc. All rights reserved.