Scaffold requirements for periodontal regeneration with enamel matrix derivative proteins
Scaffold requirements for periodontal regeneration with enamel matrix derivative proteins
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DOI：
10.1016/j.colsurfb.2017.05.013
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发表时间：
2017-08-01
影响因子：
5.8
通讯作者：
Fischer, Peter
中科院分区：
文献类型：
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作者：
Apicella, Alessandra;Heunemann, Peggy;Fischer, Peter
牙周炎影响天然牙齿的附着，与牙周炎相关的感染或炎症可能影响种植体周围组织。牙釉质基质衍生物（EMD）蛋白作为骨移植材料混合物的一部分用于骨内大面积缺损时，可刺激受损组织的自我再生。作为促进细胞增殖和韧带形成过程的第一步，我们证明了EMD蛋白沉淀在很大程度上取决于混合物中使用的骨移植物的物理和化学特性。为了保证最佳的蛋白质刺激自我调节，初始EMD配方的pH值必须在3.9和4.2之间调整，以补偿骨移植引起的pH值变化。此外，这两种组分的相互作用导致不同形状和大小的沉淀物不同地覆盖在接枝上。这一结果可能对细胞附着和牙周韧带延伸有潜在的临床意义，值得进一步的体外和体内试验。(C) 2017 Elsevier B.V.版权所有
Periodontitis affects the attachment of natural teeth, and infection or inflammation associated with periodontitis may affect peri-implant tissues. Enamel matrix derivative (EMD) proteins provide stimulation for self-regeneration of the damaged tissue when applied to wide intrabony defects as part of a mixture with bone graft material. As a first step of the process enhancing cell proliferation and ligament formation, we demonstrated that EMD protein precipitation depends strongly on the physical and chemical characteristics of the bone grafts used in the mixture. To guarantee optimum protein-stimulated self regulation, the pH of the initial EMD formulation must therefore be adjusted between 3.9 and 4.2 in order to compensate the change in pH induced by the bone graft. Moreover, the interaction between the two components resulted in precipitates of different shape and size differently covering the grafts. This outcome might potentially have clinical implications on cell attachment and periodontal ligament extension, which deserve further in vitro and in vivo tests. (C) 2017 Elsevier B.V. All rights reserved.