Effect of acute cadmium exposure on metal accumulation and oxidative stress biomarkers of Sparus aurata.
Effect of acute cadmium exposure on metal accumulation and oxidative stress biomarkers of Sparus aurata.
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DOI：
10.1016/j.ecoenv.2012.12.015
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发表时间：
2013-03
影响因子：
6.8
通讯作者：
Ghada Souid;Nouha Souayed;Fatma Yaktiti;K. Maaroufi
中科院分区：
文献类型：
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作者：
Ghada Souid;Nouha Souayed;Fatma Yaktiti;K. Maaroufi
镉（Cd）是一种非必需金属，对水生物种可能具有微量毒性。海水中镉浓度的增加主要与日益增长的工业和农业活动产生的废水沃茨有关。采用原子吸收光谱法研究了短期（2、4和24 h）暴露条件下Cd（0.5mg/L）在金鲷（Sparusaurata）不同组织中的积累情况。这项工作还研究了金属对氧化应激生物标志物和乙酰胆碱酯酶活性（AchE）的影响。我们的研究结果表明，镉在不同组织中的积累在很大程度上取决于暴露期的金属的长度。事实上，在24小时后获得最高浓度。镉在各组织中的积累顺序为：肠>肝>鳃>背肌。镉染毒24 h后，过氧化氢酶（CAT）活性、谷胱甘肽（GSH）水平和丙二醛（MDA）含量显著升高。相反，AchE活性降低后，同一时期的暴露于金属。除超氧化物歧化酶（SOD）活性在暴露后4 h达到最高水平外，氧化应激生物标志物在暴露后2和4 h均无显著变化。这些结果表明，短期暴露于Cd（0.5mg/L）可诱导金鲷肠道金属积累和显著的氧化应激反应。
Cadmium (Cd) is a non-essential metal which could be toxic in traces for aquatic species. Increasing Cd concentrations in sea water is mainly related to waste waters provided from growing industrial and agricultural activities. The present study investigated the accumulation of Cd (0.5mg/L) in different tissues of Sparus aurata under a short term exposure (2, 4 and 24h) using the atomic absorption. This work investigated also the impact of the metal on oxidative stress biomarkers and acetyl cholinesterase activity (AchE). Our results showed that Cd accumulation in different tissues depends largely on the length of the exposure period to the metal. Indeed, the highest concentrations were obtained after 24h. Cd accumulation in tissues was in the following order: intestines>liver>gills>dorsal muscle. Cadmium administration increased significantly catalase activity (CAT), glutathione level (GSH) and malondialdehyde production (MDA) after 24h of exposure. In contrast, AchE activity was decreased after the same period of exposure to the metal. There were no significant changes in oxidative stress biomarkers after 2 and 4h of exposure, except for superoxide dismutase (SOD) activity which attained the highest level after 4h. These results suggest that short-term exposure of Sparus aurata to Cd (0.5mg/L) induced an important metal accumulation in intestine and a notable oxidative stress response.