The response of primary keratinocytes and fibroblasts trom cancer patients to multiple low-dose irradiations
The response of primary keratinocytes and fibroblasts trom cancer patients to multiple low-dose irradiations
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DOI：
10.1667/rr1001.1
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发表时间：
2007-11-01
影响因子：
3.4
通讯作者：
Kojs, Zbigniew
中科院分区：
文献类型：
--
作者：
Slonina, Dorota;Biesaga, Beata;Kojs, Zbigniew
在我们以前的研究中，使用微核（MN）试验，超辐射敏感性（HRS）的现象后，观察到单次低剂量的成纤维细胞从两个和角质形成细胞从四个研究的40例患者。在本文中，我们报告了23例原发性角质形成细胞和21例这些癌症患者的成纤维细胞对多次低剂量照射的反应，并回答了关于单次低剂量照射后出现HRS样反应的患者是否也表现出多次低剂量照射后的染色体超敏反应的问题。用三个剂量的0.25戈伊照射细胞，间隔4小时，MN诱导与相同的总剂量后，作为一个单一的分数0.75戈伊。同样地，将三次0.5戈伊剂量的效果与单次1.5戈伊剂量的效果进行比较。对于来自两名患者的成纤维细胞和来自四名患者的角质形成细胞，他们表现出单次剂量的HRS样反应，观察到分级的显著相反效应（在三次0.25戈伊剂量后比在单次0.75戈伊剂量后更大的MN诱导），这表明在每次0.25戈伊剂量后重复的过敏反应。对于来自19名单剂量HRS样阴性患者的细胞，没有观察到这种效果。总之，在3次0.25戈伊剂量（但不是3 x 0.5戈伊）后，在2名患者的成纤维细胞和4名患者的角质形成细胞中观察到MN诱导的逆分馏效应，反映了在相同患者中观察到的对单次低剂量的反应的染色体超放射敏感性。
In our previous study, using the micronucleus (MN) assay, a hyper-radiosensitivity (HRS)-like phenomenon was observed after single low doses for fibroblasts from two and keratinocytes from four of the 40 patients studied. In this paper, we report the response of primary keratinocytes from 23 and fibroblasts from 21 of these cancer patients to multiple low-dose irradiations and answer the question regarding whether the patients with an HRS-like response after single low doses also demonstrate chromosomal hypersensitivity after multiple low doses. The cells were irradiated with three doses of 0.25 Gy separated by 4-h intervals, and MN induction was compared with that after the same total dose given as a single fraction of 0.75 Gy. Similarly, the effect of three doses of 0.5 Gy was compared with that of a single dose of 1.5 Gy. For fibroblasts from two and keratinocytes from four patients who demonstrated a single-dose HRS-like response, a significant inverse effect of fractionation (greater MN induction after three doses of 0.25 Gy than after a single dose of 0.75 Gy) was observed, which suggests a repeated hypersensitive response after each dose of 0.25 Gy. Such an effect was not seen for the cells from 19 patients who were single-dose HRS-like negative. In conclusion, an inverse fractionation effect for MN induction that was observed in fibroblasts from two and keratinocytes from four patients after three doses of 0.25 Gy (but not 3 x 0.5 Gy) reflects the chromosomal hyper-radiosensitivity seen in the same patients in response to single low doses.