Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species
Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species
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DOI：
10.1093/nar/26.6.1458
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发表时间：
1998-03-15
影响因子：
14.9
通讯作者：
Lagnel, J
中科院分区：
文献类型：
--
作者：
Desmarais, E;Lanneluc, I;Lagnel, J
直接扩增长度多态性（DALP）使用任意引物PCR （AP-PCR）来产生基因组指纹，并能够对几乎任何物种的DNA多态性进行测序，寡核苷酸对被设计为每个产生特定的多条带模式，因此产生的所有片段都可以用相同的两个通用M13测序引物直接测序。该策略结合了高分辨率指纹技术的优势和多态性表征的可能性，在多位点检测步骤中使用家庭成员作为模板，可以直接测试等位基因的传播，以及早期定位标记或选择与某些性状或疾病相关的位点，我们使用这种方法检测微缺失/插入和微卫星DNA位点，在群体遗传学研究中有用。但它可以应用于生物学的许多其他领域，例如基因组定位，用于定义多态序列标记位点，直接定位在遗传图谱上。
Direct amplification of length polymorphisms (DALP) uses an arbitrarily primed PCR (AP-PCR) to produce genomic fingerprints and to enable sequencing of DNA polymorphisms in virtually any species, Oligonucleotide pairs were designed to each produce a specific multi-banded pattern and all the fragments thus generated can be directly sequenced with the same two universal M13 sequencing primers, This strategy combines the advantages of a high resolution fingerprint technique and the possibility of characterizing the polymorphisms, The use of family members as templates in the multi-locus detection step allows a direct test of allele transmission, as well as early mapping of the markers or selection of loci associated with some traits or diseases, We used this method to detect micro-deletions/insertions and microsatellite DNA loci useful in population genetics studies, but it could be applied in many other fields of biology, such as genome mapping for definition of polymorphic sequence tagged sites, directly localized on a genetic map.