CLONAL GROWTH OF HUMAN PROSTATIC EPITHELIAL-CELLS IS STIMULATED BY FIBROBLASTS
CLONAL GROWTH OF HUMAN PROSTATIC EPITHELIAL-CELLS IS STIMULATED BY FIBROBLASTS
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DOI：
10.1002/pros.2990140306
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发表时间：
1989-01-01
期刊：
影响因子：
2.8
通讯作者：
STAMEY, TA
中科院分区：
文献类型：
--
作者：
KABALIN, JN;PEEHL, DM;STAMEY, TA
在体外研究了成纤维细胞与成人前列腺上皮细胞的相互作用。当前列腺上皮细胞与成纤维细胞共培养时，通过克隆生长测定测量，证明了上皮细胞增殖的刺激。与成纤维细胞共培养中的上皮生长大于在先前描述的“优化的”无血清培养基中的分离培养中获得的上皮生长。成纤维细胞能够补偿这种“优化”培养基中几种生长因子的缺失，包括表皮生长因子和胰岛素，但明显无法替代牛垂体提取物。由成纤维细胞调节的培养基产生了与共培养中相同的上皮生长刺激。使用成人前列腺来源的成纤维细胞、成人皮肤、人胎儿肺成纤维细胞 (IMR-90) 和小鼠 3T3 细胞也获得了类似的结果。来自正常或恶性组织的前列腺上皮细胞之间没有显示出反应差异。由成纤维细胞培养物制备的固定成纤维细胞单层和细胞外基质未能刺激前列腺上皮生长。这些结果表明，可溶性生长因子由前列腺成纤维细胞和其他非前列腺来源的人类成纤维细胞，以及来自非人类物种的胚胎间充质细胞分泌，其能够在体外从成人前列腺上皮产生显着的增殖反应。 通过向前列腺上皮细胞培养物中添加多种已知的生长因子，无法重现这种增殖反应。
The interaction of fibroblast with adult human prostatic epithelial cells was studied in vitro. Stimulation of epithelial cell proliferation, measured by clonal growth assay, was demonstrated when prostatic epithelial cells were grown in coculture with fibroblasts. Epithelial growth in cocultures with fibroblasts was greater than could be obtained in isolated culture in an "optimized," serum-free medium previously described. Fibroblasts were able to compensate for the deletion of several growth factors from this "optimized" medium, including epidermal growth factor and insulin, but were notably unable to replace bovine pituitary extract. Epithelial growth stimulation identical to that achieved in coculture was produced by medium conditioned by fibroblasts. Similar results were obtained by using fibroblasts of adult human prostate origin, adult human skin, human fetal lung fibroblasts (IMR-90), and mouse 3T3 cells. No difference in response was demonstrated between prostatic epithelial cells derived from normal or malignant tissues. Fixed fibroblast monolayers and extracellular matrix prepared from fibroblast cultures failed to stimulate prostatic epithelial growth. These results suggest that a soluble growth factor is secreted by prostatic fibroblasts and other human fibroblasts of nonprostatic origin, as well as by embryonic mesenchymal cells from nonhuman species, which is capable of producing a marked proliferative response in vitro from adult human prostatic epithelium. This proliferative response could not be reproduced by the addition of a variety of known growth factors to prostatic epithelial cell cultures.