Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device.
Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device.
复制标题
使用微流体装置通过基于细胞融合的方法定量控制细胞间线粒体转移。
作者：
K. Wada;K. Hosokawa;Yoshihiro Ito;M. Maeda
线粒体转移的定量控制是线粒体DNA（mtDNA）遗传操作的一种有前途的方法，因为它能够精确调节异质性。此外，从mtDNA突变累积细胞到mtDNA较少（ρ0）细胞的单克隆转移可能实现突变mtDNA的同质性。在这里，我们描述了用于定量控制线粒体转移的方法，包括使用微流体装置实现活的单细胞之间的单个线粒体转移。
Quantitative control of mitochondrial transfer is a promising approach for genetic manipulation of mitochondrial DNA (mtDNA) because it enables precise modulation of heteroplasmy. Furthermore, single mitochondrion transfer from a mtDNA mutation-accumulated cell to a mtDNA-less (ρ0) cell potentially achieves homoplasmy of mutated mtDNA. Here we describe the method for quantitative control of mitochondrial transfer including achieving single mitochondrion transfer between live single cells using a microfluidic device.