Relative efficacy of embryonated eggs and cell culture for isolation of contemporary influenza viruses
Relative efficacy of embryonated eggs and cell culture for isolation of contemporary influenza viruses
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含胚卵和细胞培养物分离当代流感病毒的相对功效
DOI：
10.1128/jcm.13.1.233-235.1981
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发表时间：
1981
影响因子：
9.4
通讯作者：
E. Bryan
中科院分区：
文献类型：
--
作者：
A. Monto;H. F. Maassab;E. Bryan
通过平行接种临床材料，在三个季节内比较了含胚卵和原代猴肾细胞培养分离当代流感病毒的效率。对于1977-1978年的A型（H1N1）毒株和B型毒株，鸡蛋的效率非常低，几乎所有的病毒都是单独在细胞培养中分离的。对于1978-1979年的A型（H1N1）毒株和A型（H3 N2）毒株，更经常在鸡蛋中进行分离，但即使在这里，鸡蛋也不如细胞培养物那样有效。结论：原代猴肾细胞培养物可用作当前A型和B型流感病毒原代分离的单一系统。
The efficiency of embryonated eggs and primary monkey kidney cell culture for isolation of contemporary influenza viruses was compared over three seasons by parallel inoculation of clinical materials. For the type A (H1N1) strains of 1977-1978 and for type B strains eggs were very inefficient, and nearly all viruses were isolated in cell culture alone. For the type A (H1N1) strains of 1978-1979 and for type A (H3N2) strains, isolations were more frequently made in eggs, but even here eggs were not as efficient for primary isolation as were cell cultures. It is concluded that primary monkey kidney cell cultures can be used as the single system for primary isolation of current type A and B influenza viruses.