Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 protein level and lipid contents in the skeletal muscle of sucrose-rich diet fed rats
Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 protein level and lipid contents in the skeletal muscle of sucrose-rich diet fed rats
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DOI：
10.1016/j.plefa.2012.11.003
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发表时间：
2013-02-01
影响因子：
3
通讯作者：
Lombardo, Yolanda B.
中科院分区：
文献类型：
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作者：
D'Alessandro, Maria E.;Chicco, Adriana;Lombardo, Yolanda B.
n - 3多不饱和脂肪酸（PUFA）预防/逆转SRD诱导的肌肉胰岛素抵抗（IR）的作用和潜在机制尚未完全了解。因此，我们检查了：甘油三酯、二酰基甘油、PKC θ、Glut-4、酶促己糖激酶活性、IRS-1蛋白质质量水平和肌肉磷脂的脂肪酸组成。大鼠在6个月内喂食SRD。此后，一半的动物继续SRD长达8个月;另一半喂食SRD，其中CO（8%wt/wt）被FO（7%+1%CO）替代2个月。结果进行了比较，在大鼠喂食对照饮食（CD）。在SRD喂养的大鼠中，FO油使脂质储存和PKC θ蛋白质质量水平正常化/改善。胰岛素的作用与CD喂养的大鼠相当。FO逆转受损的葡萄糖磷酸化，IRS-1，并在胰岛素刺激下，Glut-4蛋白质质量水平。FO使胰岛素抵抗正常化，并增加肌肉磷脂中的n - 3 PUFA。(c)2012爱思唯尔有限公司保留所有权利。
The role and underlying mechanisms by which n - 3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKC theta, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKC theta protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n - 3 PUFAs in muscle phospholipids. (c) 2012 Elsevier Ltd. All rights reserved.