In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells
In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells
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DOI：
10.1016/j.bbrc.2005.12.047
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发表时间：
2006-02-10
影响因子：
3.1
通讯作者：
Soleimani, M
中科院分区：
文献类型：
--
作者：
Kadivar, M;Khatami, S;Soleimani, M
缺血性心脏损伤后，心肌细胞的丢失往往导致不可逆的心功能缺陷。间充质干细胞是一种多能细胞，在适当的刺激下可以分化为特殊的细胞，近年来，间充质干细胞的细胞心肌成形术已成为修复受损心肌的一种新方法。本研究旨在探讨人脐带间充质干细胞向具有心肌细胞特征的细胞分化的潜能。使用与人脐静脉内皮细胞分离类似的方法从人脐带的内皮/内皮下层分离间充质干细胞。分离的细胞的特点是转分化成脂肪细胞和成骨细胞的能力，并与流式细胞仪分析。经5-氮胞苷处理后，人脐带间充质干细胞在形态上转化为心肌样细胞，并表达心肌分化标志物。在分化过程中，通过相差显微镜监测细胞并显示其形态学变化。肌节肌球蛋白（MF 20），结蛋白，心肌肌钙蛋白I和肌节肌动蛋白的分化细胞的免疫染色呈阳性。RT-PCR分析表明，这些分化的细胞表达心脏特异性基因。透射电镜下可见心肌样超微结构和典型的肌角。这些观察结果证实了人脐带来源的间充质干细胞可以化学转化为心肌细胞，并且可以被认为是细胞心肌成形术的细胞来源。(c)2005年爱思唯尔公司All rights reserved.
Cardiomyocyte loss in the ischemically injured human heart often leads to irreversible defects in cardiac function. Recently, cellular cardiomyoplasty with mesenchymal stem cells, which are multipotent cells with the ability to differentiate into specialized cells under appropriate stimuli, has emerged as a new approach for repairing damaged myocardium. In the present study, the potential of human umbilical cord-derived mesenchymal stem cells to differentiate into cells with characteristics of cardiomyocyte was investigated. Mesenchymal stem cells were isolated from endothelial/subendothelial layers of the human umbilical cords using a method similar to that of human umbilical vein endothelial cell isolation. Isolated cells were characterized by transdifferentiation ability to adipocytes and osteoblasts, and also with flow cytometry analysis. After treatment with 5-azacytidine, the human umbilical cord-derived mesenchymal stem cells were morphologically transformed into cardiomyocyte-like cells and expressed cardiac differentiation markers. During the differentiation, cells were monitored by a phase contrast microscope and their morphological changes were demonstrated. Immunostaining of the differentiated cells for sarcomeric myosin (MF20), desmin, cardiac troponin I, and sarcomeric chi-actinin was positive. RT-PCR analysis showed that these differentiated cells express cardiac-specific genes. Transmission electron microscopy revealed a cardiomyocyte-like ultrastructure and typical sarcomers. These observations confirm that human umbilical cord-derived mesenchymal stem cells can be chemically transformed into cardiomyocytes and can be considered as a source of cells for cellular cardiomyoplasty. (c) 2005 Elsevier Inc. All rights reserved.