A common genetic basis in sweet corn inbred Cr1 for cross sensitivity to multiple cytochrome P450-metabolized herbicides
A common genetic basis in sweet corn inbred Cr1 for cross sensitivity to multiple cytochrome P450-metabolized herbicides
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DOI：
10.1614/ws-07-145.1
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发表时间：
2008-05-01
期刊：
影响因子：
2.5
通讯作者：
Lutz, Joseph D.
中科院分区：
文献类型：
--
作者：
Nordby, Jonathan N.;Williams, Martin M., II;Lutz, Joseph D.
烟嘧磺隆、甲基磺草酮、麦草畏加二氟吡唑和唑草酮是来自不同化学家族的具有不同作用模式的芽后除草剂。在温室试验中，对来自Cr1（敏感自交系）和Cr2（耐受自交系）杂交的143个F（3：4）家系（F（4）植株）进行了评价，观察到甜玉米对这些除草剂的敏感性之间的关联。耐受率：分离：敏感家系与3：2：3的比例没有显著差异，如果单基因调节除草剂反应，这将是预期的。家庭共分离这些除草剂的反应。在2005年和2007年分别对60个F（3：5）家系和120个F（3：5）家系进行的田间研究中，对这些除草剂和甲酰胺磺隆和乙嘧磺隆的反应是相关的。在田间试验中，苯达松的耐受性家庭的上述除草剂的反应是相似的，但注意到差异的家庭是敏感的或分离的响应烟嘧磺隆，甲酰胺磺隆，双嘧磺隆，甲基磺草酮，麦草畏加diflufenzopyr，和唑草酮。影响Cr1中除草剂敏感性的基因与先前测序的细胞色素P450基因位于染色体5S的相同区域，其中先前指定的等位基因nsd 1和ben 1与烟嘧磺隆和苯达松的敏感性相关，似乎是392碱基对插入突变的结果。本研究支持了甜玉米自交系Cr1中的单隐性基因或紧密连锁基因对多种细胞色素P450酶代谢除草剂敏感的假设。
Nicosulfuron, mesotrione, dicamba plus diflufenzopyr, and carfentrazone are postemergence herbicides from different chemical families with different modes of action. An association between the sensitivity of sweet corn to these herbicides was observed when 143 F(3:4) families (F(4) plants) derived from of a cross between Cr1 (sensitive inbred) and Cr2 (tolerant inbred) were evaluated in greenhouse trials. The ratio of tolerant : segregating : sensitive families was not significantly different from a 3 : 2 : 3 ratio, which would be expected if a single gene conditioned herbicide response. Families cosegregated for responses to these herbicides. In field studies with 60 F(3 : 5) families in 2005 and 120 F(3 : 5) families in 2007, responses to these herbicides and foramsulfuron and primisulfuron were associated. Responses to bentazon in field trials were similar to the aforementioned herbicides for tolerant families, but differences were noted for families that were sensitive or segregated for responses to nicosulfuron, foramsulfuron, primisulfuron, mesotrione, dicamba plus diflufenzopyr, and carfentrazone. The gene(s) affecting herbicide sensitivity in Cr1 maps to the same region of chromosome 5S as a previously sequenced cytochrome P450 gene, where alleles previously designated nsd1 and ben1 were associated with sensitivity to nicosulfuron and bentazon and appear to be the result of a 392-base-pair insertion mutation. This work supports the hypothesis that a single recessive gene or closely linked genes in the sweet corn inbred Cr1 condition sensitivity to multiple cytochrome P450 enzyme-metabolized herbicides.