Small bowel preservation using a cavitary two-layer (University of Wisconsin solution/perfluorochemical) cold storage method.
Small bowel preservation using a cavitary two-layer (University of Wisconsin solution/perfluorochemical) cold storage method.
复制标题
使用空腔两层（威斯康星大学溶液/全氟化学品）冷藏方法保存小肠。
作者：
Y. Kuroda;T. Sakai;Yasuyuki Suzuki;Y. Tanioka;S. Matsumoto;Yongsik Kim;H. Fujita;M. Hamano;Y. Hasegawa;Y. Ku;Y. Saitoh
在大鼠异位节段性小肠移植模型中，评价了两层空腔(威斯康星大学溶液[UW]/全氟化学)冷冻法保存小肠的效果。简单冷藏UW仅对24小时保存有效。然而，使用UW的空腔双层方法使保存时间延长到48小时成为可能。此外，在没有氧气鼓泡的情况下，空化两层法不能有效地保存48小时。移植后7d，空腔二层法保存48h的移植物组织学检查显示，肠粘膜结构正常。本研究表明，在大鼠异位节段性小肠移植模型中，使用UW的空腔双层法保存期间小肠的氧合使保存时间延长至48小时。
Preservation of the small bowel by a cavitary two-layer (University of Wisconsin solution [UW]/perfluorochemical) cold storage method was evaluated in the heterotopic rat segmental small bowel transplant model. Simple cold storage UW was effective only for 24-hr preservation. However, the cavitary two-layer method using UW made it possible to prolong preservation time up to 48 hr. Furthermore, without oxygen bubbling, the cavitary two-layer method was not effective for 48-hr preservation. Histologic studies of grafts preserved by the cavitary two-layer method for 48 hr at 7 days after transplantation showed normal architecture of the intestinal mucosa. This study demonstrates that the oxygenation of the small bowel during preservation by the cavitary two-layer method using UW makes it possible to extend preservation time up to 48 hr in the heterotopic rat segmental small bowel transplant model.
影响因子：
5.2
作者：
Abu-Elmagd,K;Todo,S;Tzakis,A;Reyes,J;Nour,B;Furukawa,H;Fung,JJ;Demetris,A;Starzl,TE
通讯作者：
Starzl,TE