Molecular detection of porcine kobuviruses in Italian swine
Molecular detection of porcine kobuviruses in Italian swine
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DOI：
10.1016/j.rvsc.2013.06.020
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发表时间：
2013-10-01
影响因子：
2.4
通讯作者：
Di Martino, Barbara
中科院分区：
文献类型：
--
作者：
Di Profio, Federica;Ceci, Chiara;Di Martino, Barbara
本研究采用柯布病毒通用引物对采自意大利6~10月龄健康猪的260份粪便标本进行RT-PCR筛查。检测样本中检出猪柯布病毒(PKV)的比例为3.85%。根据对部分3D基因的分析，这里发现的意大利序列与之前在日本、泰国、韩国、中国和巴西发现的PKV的亲缘关系比与最近在匈牙利和荷兰发现的欧洲PKV的亲缘关系更近。(C)2013爱思唯尔有限公司。保留所有权利。
In this study, fecal specimens (n = 260) collected from Italian healthy pigs aged between 6 and 10 months were screened by RT-PCR using generic kobuvirus primers. Porcine kobuviruses (PKVs) were detected in 3.85% of the samples tested. Based on the analysis of the partial 3D gene, the Italian sequences identified here were more closely related to PKVs previously identified in Japan, Thailand, Korea, China and Brazil than to the European PKVs recently detected in Hungary and in the Netherlands. (C) 2013 Elsevier Ltd. All rights reserved.