Catecholamines inhibit the antigen-presenting capability of epidermal Langerhans cells
Catecholamines inhibit the antigen-presenting capability of epidermal Langerhans cells
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DOI：
10.4049/jimmunol.168.12.6128
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发表时间：
2002-06-15
影响因子：
4.4
通讯作者：
Granstein, RD
中科院分区：
文献类型：
--
作者：
Seiffert, K;Hosoi, J;Granstein, RD
交感神经系统在多个水平上调节免疫功能。在表皮内，朗格汉斯细胞(LC)在解剖上常与周围神经联系。此外，一些神经肽已被证明对LC抗原提呈功能具有调节作用。我们探讨了肾上腺素能受体(AR)在小鼠LC中的表达，并评价了AR在抗原提呈和调节皮肤免疫反应中的功能作用。纯化的LC和类LC细胞系XS524D和XS106均表达Arsα(1A)和β(2)的mRNA。XS106细胞和纯化的LC也表达β(1)-AR mRNA。用肾上腺素(EPI)或去甲肾上腺素处理小鼠表皮细胞制剂可抑制体外的抗原提呈。此外，用EPI或去甲肾上腺素在体外对表皮细胞进行预处理，可抑制这些细胞呈递抗原的能力，从而在先前免疫的小鼠中诱发迟发性超敏反应。这种作用可被β(2)-肾上腺素能拮抗剂ICI 118,551阻断，但不能被α-受体拮抗剂酚妥拉明阻断。局部皮内注射EPI可抑制皮下注射半抗原引起的接触性超敏反应。令人惊讶的是，在远处注射EPI也抑制了接触性超敏反应的诱导。因此，儿茶酚胺可能对局部和全身都有影响。我们的结论是，LC上表达特定的AR，通过这些受体传递信号可以减少表皮免疫反应。
The sympathetic nervous system modulates immune function at a number of levels. Within the epidermis, APCs (Langerhans cells (LC) are frequently anatomically associated with peripheral nerves. Furthermore, some neuropeptides have been shown to regulate LC Ag-presenting function. We explored the expression of adrenergic receptors (AR) in murine LC and assessed their functional role on Ag presentation and modulation of cutaneous immune responses. Both purified LC and the LC-like cell lines XS524D and XS106 expressed mRNA for the ARs alpha(1A) and beta(2). XS106 cells and purified LC also expressed beta(1)-AR mRNA. Treatment of murine epidermal cell preparations with epinephrine (EPI) or norepinephrine inhibited Ag presentation in vitro. Furthermore, pretreatment of epidermal cells with EPI or norepinephrine in vitro suppressed the ability of these cells to present Ag for elicitation of delayed-type hypersensitivity in previously immunized mice. This effect was blocked by use of the beta(2)-adrenergic antagonist ICI 118,551 but not by the a-antagonist phentolamine. Local intradermal injection of EPI inhibited the induction of contact hypersensitivity to epicutaneously administered haptens. Surprisingly, injection of EPI at a distant site also suppressed induction of contact hypersensitivity. Thus, catecholamines may have both local and systemic effects. We conclude that specific ARs are expressed on LC and that signaling through these receptors can decrease epidermal immune reactions.