Enterically Induced Immunologic Tolerance
Enterically Induced Immunologic Tolerance
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肠道诱导的免疫耐受
作者：
L. K. Richman;J. Chiller;W. Brown;D. Hanson;N. Vaz
给近交系小鼠(BDF1)经胃插管注射可溶性卵清蛋白(OVA)，诱导产生特异性免疫无反应。当给动物喂食自体载体(OVA或锁孔帽状血蓝蛋白)时，抗半抗原(DNP)反应同样被特异性地减弱。脾细胞过继转移实验表明，受照小鼠可维持耐受状态，并可将特异性无能转移给正常受鼠。过继抑制是由T淋巴细胞介导的，尼龙羊毛分级和细胞对抗Thy 1.2和补体的敏感性证明了这一点。被转移的B细胞既没有抑制作用，也没有增强作用。肠道注射OVA也能特异性地减少抗原诱导的淋巴T细胞的DNA合成，尽管在淋巴中本身并没有发现抑制细胞。
Specific immune unresponsiveness was induced in inbred mice (BDF1) by the administration of soluble ovalbumin (OVA) by gastric intubation. Anti-hapten (DNP) responses likewise were specifically diminished when animals were fed autologous carrier (OVA or keyhole limpet hemocyanin). Adoptive transfer of spleen cells demonstrated that the tolerant state could be maintained in irradiated recipient mice, and specific anergy could be transferred to normal recipient animals. Adoptive suppression was mediated by T lymphocytes, as demonstrated by nylon wool fractionation and susceptibility of the cells to anti-Thy 1.2 and complement. Transferred B cells had neither suppressive nor augmentative effects. Enteric administration of OVA also specifically diminished antigen-induced DNA synthesis of primed lymph node T cells, although suppressor cells were not identified in the lymph nodes per se.