Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
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DOI：
10.1007/978-1-60327-017-5_25
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Zhou, Zheng
中科院分区：
文献类型：
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作者：
Lu, Nan;Yu, Xiaomeng;Zhou, Zheng
细胞凋亡是细胞自杀的基因控制过程，在动物发育和维持体内平衡中发挥重要作用。线虫秀丽隐杆线虫已被证明是在细胞生物学和遗传工具的辅助下研究控制细胞凋亡和随后的细胞凋亡细胞清除的机制的优秀模型生物。特别是，线虫身体和蛋壳的透明性质使得线虫特别适合活细胞显微镜观察。在这里，我们描述了一些识别活体秀丽隐杆线虫胚胎和成虫中凋亡细胞并监测其在胚胎发育过程中清除的方法。这些方法基于微分干涉对比显微镜和使用基于 GFP 的报告基因的荧光显微镜。
Apoptosis is a genetically controlled process Of Cell suicide that plays an important role in animal development and in maintaining homeostasis. The nematode Caenorhabditis elegans has proven to be all excellent model organism for Studying the mechanisms controlling apoptosis and the subsequent clearance of apoptotic cells, aided with cell-biological and genetic tools. In particular, the transparent nature of worm bodies and eggshells makes C. elegans particularly amiable for live cell microscopy. Here we describe a few methods for identifying apoptotic cells in living C. elegans embryos and adults and for monitoring their clearance during embryonic development. These methods arc based on Differential Interference Contrast microscopy and on fluorescence microscopy using GFP-based reporters.