Effects of 120- to 165-nm vacuum-uv light on wet yeast cells.
Effects of 120- to 165-nm vacuum-uv light on wet yeast cells.
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120 至 165 nm 真空紫外光对湿酵母细胞的影响。
DOI：
10.2307/3575446
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发表时间：
1981
影响因子：
3.4
通讯作者：
A. Ito
中科院分区：
文献类型：
--
作者：
T. Ito;A. Ito
利用带有MgF 2出射窗的氘灯，研究了真空紫外区短波长光对湿酵母细胞的影响。真空紫外线（120-165 nm）灭活细胞主要是通过表面附近的损伤，可能是在细胞质膜。伴随的遗传效应归因于远紫外线组件的基础上的光复活的证据和波长的考虑，使用截止滤波器的结果。失活没有被光活化，也没有发生时，SiO2过滤器被插入在光束路径，切断波长短于165 nm。真空紫外光通过非DNA损伤的细胞灭活的能力进一步证明了在失活率的下降和真空紫外线（120- 165-nm）强度的衰减在实验中，出射窗口和细胞样品之间的水蒸气的厚度被改变之间的相关性。这些发现是consi…
The effects of short wavelength light on wet yeast cells were studied with special reference to the vacuum-uv region using a deuterium discharge lamp with ${\rm MgF}_{2}$ exit window. Vacuum uv (120-165 nm) inactivated the cells mainly through damage inflicted near the surface, presumably in the cytoplasmic membrane. Concomitant genetic effects were ascribed to the far-uv component of the emission based on the photoreactivation evidence and wavelength considerations using results with cutoff filters. The inactivation was not photoreactivated, nor did it occur when a SiO2 filter was inserted in the beam path, which cut off wavelengths shorter than 165 nm. The capability of vacuum-uv light for cell inactivation via non-DNA damage was further demonstrated by the correlation between the decrease in the inactivation rate and the attenuation of the vacuum-uv (120- to 165-nm) intensity in experiments where the thickness of water vapor between the exit window and cell samples was changed. These findings are consi...