BINDING OF ACTINOMYCES-VISCOSUS TO COLLAGEN - ASSOCIATION WITH THE TYPE-1 FIMBRIAL ADHESIN
BINDING OF ACTINOMYCES-VISCOSUS TO COLLAGEN - ASSOCIATION WITH THE TYPE-1 FIMBRIAL ADHESIN
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DOI：
10.1111/j.1399-302x.1991.tb00443.x
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发表时间：
1991-02-01
影响因子：
--
通讯作者：
SKOBE, Z
中科院分区：
文献类型：
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作者：
LIU, T;GIBBONS, RJ;SKOBE, Z
用人I型或III型胶原处理羟基磷灰石(HA)可显著促进粘性放线菌LY7细胞的黏附。人V型胶原的治疗效果较差，而人IV型胶原或大鼠I型胶原的治疗效果明显较差。电子显微镜观察显示粘性支原体细胞也附着在由人I型胶原制备的纤维上。来源于I型胶原的α1(1)多肽链没有促进结合的作用，而α2(1)多肽链表现出中等的活性。热变性或尿素变性的I型胶原在促进结合方面也没有效果。粘液曲霉的突变体具有1型菌毛，但没有2型菌毛或没有菌毛，也与胶原处理的HA结合；这表明负责的粘附素与1型菌毛有关。以色列放线菌和齿状放线菌对胶原处理的HA也表现出很强的结合能力，而内氏放线菌ATCC 12104则没有。放线菌对胶原蛋白的亲和力似乎是在牙骨质和牙根表面发现高比例放线菌的最小部分原因。
Treatment of hydroxyapatite (HA) with human type I or type III collagen strongly promoted adhesion of Actinomyces viscosus LY7 cells. Treatment with human type V collagen was somewhat less effective while treatment with human type IV or rat type I collagen was significantly less effective. Electron microscopic observations revealed that A. viscosus cells also attached to fibrils prepared from human type I collagen. The alpha 1 (1) polypeptide chain derived from type I collagen was ineffective in promoting binding and the alpha 2 (1) polypeptide chain exhibited moderate activity. Heat- or urea-denatured type I collagens were also ineffective in promoting binding. Mutants of A. viscosus that possess type 1 fimbriae, but not type 2 fimbriae or no fimbriae, also bound to collagen-treated HA; this suggests that the adhesin responsible was associated with type 1 fimbriae. Strains of Actinomyces israelii and Actinomyces odontolyticus also exhibited strong binding to collagen-treated HA, while Actinomyces naeslundii ATCC 12104 did not. The avidity of Actinomyces species for collagen would seem to be least partially responsible for the high proportions of these organisms found on cemental and root tooth surfaces.