Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy
Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy
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DOI：
10.1093/ndt/gfw343
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发表时间：
2017-12-01
影响因子：
6.1
通讯作者：
Narita, Ichiei
中科院分区：
文献类型：
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作者：
Watanabe, Hirofumi;Goto, Shin;Narita, Ichiei
背景资料。免疫球蛋白A肾病(IgAN)是最常见的慢性肾小球疾病，尤其是在扁桃体或肠道引起的粘膜免疫反应参与了疾病的发生发展。为了探讨IgAN与扁桃体内细菌菌群的关系，我们进行了全面的微生物组分析。我们纳入了48例IgAN患者，21例复发性扁桃体炎(RT)患者和30例扁桃体增生症(TH)儿童，他们都曾接受过扁桃体切除术。从每个患者的扁桃体隐窝提取基因组DNA，并扩增16S核糖体RNA基因V4区，采用高通量多重测序方法进行分析。用排列多变量方差分析和主成分分析(PCA)对3个研究组的属组成差异进行统计分析。在每个样本中都检测到了细菌组成的大量多样性。Prevotala spp.、梭杆菌属Fusbacterium spp.、鞘氨醇单胞菌属Sphingomonas spp.和密螺旋体(Treponema spp.)在IgAN患者中占主导地位。普雷沃特氏菌、嗜血杆菌、卟啉单胞菌的丰度百分比。和密螺旋体(Treponema spp.)IgAN组与TH组比较，差异有统计学意义。然而，在IgAN和RT患者中，任何细菌属的丰度百分比都没有显著差异。PCA虽能区分IgAN和RT，但不能区分IgAN和RT。不同临床病理参数的IgA肾病患者的微生物群组成差异无统计学意义。在IgAN和RT患者的扁桃体隐窝中都存在相似的细菌模式，提示宿主对这些细菌的反应可能在IgAN的发生发展中起重要作用。
Background. Immunoglobulin A nephropathy (IgAN) is the most prevalent primary chronic glomerular disease, in which the mucosal immune response elicited particularly in the tonsils or intestine has been estimated to be involved in the development of the disease. To explore the relationship between IgAN and bacterial flora in the tonsils, we conducted a comprehensive microbiome analysis.Methods. We enrolled 48 IgAN patients, 21 recurrent tonsillitis (RT) patients without urine abnormalities and 30 children with tonsillar hyperplasia (TH) who had undergone tonsillectomy previously. Genomic DNA from tonsillar crypts of each patient was extracted, and V4 regions of the 16S ribosomal RNA gene were amplified and analysed using a high-throughput multiplexed sequencing approach. Differences in genus composition among the three study groups were statistically analysed by permutational multivariate analysis of variance and visualized by principal component analysis (PCA).Results. Substantial diversity in bacterial composition was detected in each sample. Prevotella spp., Fusobacterium spp., Sphingomonas spp. and Treponema spp. were predominant in IgAN patients. The percentage of abundance of Prevotella spp., Haemophilus spp., Porphyromonas spp. and Treponema spp. in IgAN patients was significantly different from that in TH patients. However, there was no significant difference in the percentage of abundance of any bacterial genus between IgAN and RT patients. PCA did not distinguish IgAN from RT, although it discriminated TH. No significant differences in microbiome composition among the groups of IgAN patients according to clinicopathological parameters were observed.Conclusions. Similar patterns of bacteria are present in tonsillar crypts of both IgAN and RT patients, suggesting that the host response to these bacteria might be important in the development of IgAN.