Design and Synthesis of a Fluorescently End-Labeled Poly(β-amino ester): Application to the Characterization of Degradable Polyelectrolyte Multilayers
Design and Synthesis of a Fluorescently End-Labeled Poly(β-amino ester): Application to the Characterization of Degradable Polyelectrolyte Multilayers
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DOI：
10.1002/pola.24578
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发表时间：
2011-04-01
影响因子：
--
通讯作者：
Lynn, David M.
中科院分区：
文献类型：
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作者：
Bechler, Shane L.;Lynn, David M.
我们报告的合成的荧光末端标记的类似物的合成和可降解的阳离子聚（β-氨基酯）（PBAE;聚合物1）在过去的研究中使用的交付的DNA和可侵蚀的多层膜（PEM）的逐层组装。具有丙烯酸酯官能化端基的聚合物1的类似物的合成提供了通过胺官能化荧光团的聚合后共轭加成引入荧光标记的平台。该方法使得能够合成荧光末端标记的聚合物（聚合物1（FL）），其分子量和多分散性（M-n = 18，000; PDI类似于1.8）类似于过去研究中使用聚合物1制造PEM所用的那些。层-层组装的PEM使用聚合物1FL和聚（苯乙烯磺酸盐），使表征膜侵蚀，并首次，直接观察阳离子聚合物从这些组件使用荧光显微镜和荧光测定法的释放。我们的研究结果揭示了这些PEM的阳离子组分的行为，并可能被证明是有用的一系列不同的控制释放应用的薄膜的设计。我们的研究结果还提供了新的荧光阳离子聚合物探针，可以用于表征PBAE在其他基础或应用生物技术背景下的行为。(C)2011 Wiley Periodicals，Inc.聚合物科学杂志A部分：聚合物化学49：1572-1581，2011年
We report the synthesis of a fluorescently end-labeled analog of a synthetic and degradable cationic poly(beta-amino ester) (PBAE; polymer 1) used in past studies for the delivery of DNA and the layer-by-layer assembly of erodible polyelectrolyte multilayers (PEMs). The synthesis of an analog of polymer 1 having acrylate-functionalized end groups provided a platform for the introduction of fluorescent labels by postpolymerization conjugate addition of amine-functionalized fluorophores. This approach enabled the synthesis of fluorescently end-labeled polymer (polymer 1(FL)) with molecular weights and polydispersities (M-n = 18,000; PDI similar to 1.8) similar to those used in past studies for the fabrication of PEMs using polymer 1. Layer-by-layer assembly of PEMs using polymer 1FL and poly(styrene sulfonate) enabled characterization of film erosion and, for the first time, direct observation of the release of cationic polymer from these assemblies using fluorescence microscopy and fluorometry. Our results shed new light on the behaviors of the cationic components of these PEMs and could prove useful for the design of thin films for a range of different controlled release applications. Our results also provide new fluorescent cationic polymer probes that could be useful for characterization of the behaviors of PBAEs in other fundamental or applied biotechnological contexts. (C) 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 49: 1572-1581, 2011