Real Time Noninvasive Monitoring of Contaminating Bacteria in a Soft Tissue Implant Infection Model
Real Time Noninvasive Monitoring of Contaminating Bacteria in a Soft Tissue Implant Infection Model
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DOI：
10.1002/jbm.b.31158
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发表时间：
2009-01-01
影响因子：
3.4
通讯作者：
van Dam, Gooitzen M.
中科院分区：
文献类型：
--
作者：
Engelsman, Anton F.;van der Mei, Henny C.;van Dam, Gooitzen M.
感染是生物材料相关失效的主要原因。目前还没有一种在体内测试新型抗菌和/或非粘附性植入物涂层的简单技术。目前研究细菌在生物材料表面黏附和生长的体外方法缺乏宿主免疫系统的影响。大多数体内研究生物材料相关感染的方法通常涉及植入物移除，从而阻碍了全面的纵向监测。活体成像绕过了这些缺点，基于对生物发光细菌的非侵入性光学成像。金黄色葡萄球菌Xen29通过在其染色体上引入一个修饰的完整lux操纵子而被转基因以稳定的生物发光。将带有粘附性金黄色葡萄球菌Xen29的外科网状物植入小鼠体内，用高灵敏度的CCD摄像机通过生物发光纵向监测细菌的生长和扩散到周围组织。观察到不同的时空生物发光模式，从网状区延伸到周围组织。10天后，从移植的网状物中分离出的活生物体的数量被发现与动物牺牲前的生物发光有关。因此，使用生物发光细菌的活体成像非常适合于研究考虑宿主免疫系统的抗菌涂层。此外，对一只动物的感染进行纵向监测将显著减少实验和动物的数量。(C)2008年威利期刊公司生物材料资源B部分：APPL Biomater 88B：123-129,2009
Infection is the main cause of biomaterials-related failure. A simple technique to test in-vivo new antimicrobial and/or nonadhesive implant coatings is unavailable. Current in vitro methods for studying bacterial adhesion and growth on biomaterial surfaces lack the influence of the host immune system. Most in vivo methods to study biomaterials-related infections routinely involve implant-removal, preventing comprehensive longitudinal monitoring. In vivo imaging circumvents these drawbacks and is based on the use of noninvasive optical imaging of bioluminescent bacteria. Staphylococcus aureus Xen29 is genetically modified to be stably bioluminescent, by the introduction of a modified full lux operon onto its chromosome. Surgical meshes with adhering S. aureus Xen29 were implanted in mice and bacterial growth and spread into the surrounding tissue was monitored longitudinally from bioluminescence with a highly sensitive CCD camera. Distinct spatiotemporal bioluminescence patterns, extending beyond the mesh area into surrounding tissues were observed. After 10 days, the number of living organisms isolated from explanted meshes was found to correlate with bioluminescence prior to sacrifice of the animals. Therefore, it is concluded that in vivo imaging using bioluminescent bacteria is ideally suited to study antimicrobial coatings taking into account the host immune system. In addition, longitudinal monitoring of infection in one animal will significantly reduce the number of experiments and animals. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 88B: 123-129, 2009