Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2
Correlation between MR-spectroscopic rat hippocampal choline levels and phospholipase A2
复制标题
磁共振波谱法大鼠海马胆碱水平与磷脂酶A2的相关性
DOI：
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发表时间：
2006
影响因子：
3.1
通讯作者：
F. Henn
中科院分区：
文献类型：
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作者：
A. Sartorius;P. Schloss;B. Vollmayr;G. Ende;C. Neumann‐Haefelin;M. Hoehn;F. Henn
1H磁共振光谱（MRS）测定的海马含胆碱化合物（Cho）在重度抑郁症发作时减少，抗抑郁电休克治疗（ECT）后恢复到基线水平。电休克（ECS）是一种类似于人类ECT的电击，在大鼠身上观察到海马Cho的升高。胆碱的产生涉及多种磷脂酶的活性。为了研究Cho的增加是否与磷酸化酶A2 （PLA2）的表达增强相关，我们从MRS体素区域取矩形组织样本进行免疫印迹。我们的数据显示PLA2蛋白水平与MRS Cho/NAA水平之间存在显著的负相关（p=0.018），这表明PLA2的下调可能补偿了其他磷脂酶的上调。
Hippocampal choline-containing compounds (Cho) determined with 1H MR spectroscopy (MRS) are decreased in major depression episodes and return to baseline levels after antidepressive electroconvulsive therapy (ECT). A rise in hippocampal Cho has been observed in rats upon electroconvulsive shocks (ECS), an analogue of human ECT. Choline production involves the activity of various phospholipases. In order to investigate whether the increase of Cho correlates with an enhanced expression of phosphoslipase A2 (PLA2) we took rectangular tissue samples from the region of the MRS voxel for immunoblotting. Our data show a significant inverse correlation (p=0.018) between PLA2 protein levels and MRS Cho/NAA levels suggesting a possible downregulation of PLA2 in compensation for an upregulation of other phospholipases.
DOI：
10.1176/ajp.156.12.1895
发表时间：
1999-12
期刊：
The American journal of psychiatry
影响因子：
--
作者：
G. Pandey;Yogesh K. Dwivedi;S. Pandey;S. Teas;R. Conley;R. Roberts;C. Tamminga
通讯作者：
G. Pandey;Yogesh K. Dwivedi;S. Pandey;S. Teas;R. Conley;R. Roberts;C. Tamminga