Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo
Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo
复制标题
DOI：
10.1016/j.bbrc.2005.04.135
复制
发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Zhao, RCH
中科院分区：
文献类型：
--
作者：
Cao, Y;Sun, Z;Zhao, RCH
在这项研究中，我们从脂肪组织中分离出CD 31（-）、CD 34（-）、CD 106（-）（VCAM-1（-））和胎肝激酶（+）（Flk 1（+））细胞。这些细胞在体外可被诱导分化为成骨细胞和成脂细胞，被称为脂肪来源的成体干细胞（ADAS细胞）。我们还发现它们具有内皮祖细胞的特征。在体外，ADAS细胞表达内皮标记物时，与VEGF培养。在体内，ADAS细胞可以响应于局部线索分化成内皮细胞，这有助于后肢缺血模型中的新血管生成。PI 3激酶抑制剂LY 294002在体外阻断ADAS细胞向内皮细胞的分化。由于ADAS细胞可以在培养中扩增超过20次群体倍增而没有明显衰老，因此它们可能是细胞促血管生成疗法的潜在内皮细胞来源。(C)2005年爱思唯尔公司All rights reserved.
In this study, we isolated CD31(-), CD34(-), CD106(-) (VCAM-1(-)), and fetal liver kinase(+) (Flk1(+)) cells from adipose tissue. These cells can be induced to differentiate into cells of osteogenic and adipogenic lineages in vitro and were termed adipose derived adult stem cells (ADAS cells). We also showed that they have characteristics of endothelial progenitor cells. In vitro, ADAS cells expressed endothelial markers when cultured with VEGF. In vivo, ADAS cells can differentiate in response to local cues into endothelial cells that contributed to neoangiogenesis in hindlimb ischemia models. PI3 kinase inhibitor LY294002 blocked the differentiation of ADAS cells into endothelial cells in vitro. Because ADAS cells can be expanded in culture without obvious senescence for more than 20 population doublings, they may be a potential source of endothelial cells for cellular pro-angiogenic therapies. (C) 2005 Elsevier Inc. All rights reserved.