Changes of diurnal rhythm and levels of total and free testosterone secretion from pre to late puberty in boys: testis size of 3 ml is a transition stage to puberty
Changes of diurnal rhythm and levels of total and free testosterone secretion from pre to late puberty in boys: testis size of 3 ml is a transition stage to puberty
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DOI：
10.1530/eje.0.1510747
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发表时间：
2004-12-01
影响因子：
5.8
通讯作者：
Norjavaara, E
中科院分区：
文献类型：
--
作者：
Ankarberg-Lindgren, C;Norjavaara, E
目的：确定青春期前男孩以及整个青春期发育过程中24小时血清总睾酮和游离睾酮的比较水平。
设计：研究对象为55名健康男孩，年龄在5.0 - 18.6岁之间，他们在青春期发育过程中进行了一次或多次系列采样。
方法：用睾丸测量器测定睾丸体积。采用改良的放射免疫分析法（检测限为0.03 nmol/L）测定血清睾酮。使用基于质量作用定律推导的公式计算游离睾酮（计算游离睾酮）。
结果：在睾丸体积为1 ml至2 ml、2 ml至3 ml、6 ml至8 ml以及10 ml至15 ml之间，男孩的睾酮和计算游离睾酮浓度显著增加。睾丸体积在3 ml、4 ml、5 ml和6 ml之间未发现差异，8 ml和10 ml之间以及15 ml、20 ml和25 ml之间也未发现差异。已达到最终身高的男孩比具有相同青春期发育但未达到最终身高的男孩计算游离睾酮值更高。根据研究结果，青春期被分为六个阶段：前1期（睾丸，1 ml）、前2期（睾丸，2 ml）、早期（睾丸，3 - 6 ml）、中期（睾丸，8 - 12 ml）、晚期1期（睾丸，15 - 25 ml，未达到最终身高）和晚期2期（睾丸，15 - 25 ml，达到最终身高）。青春期前和青春期期间血清睾酮呈昼夜节律分泌。青春期男孩早晨睾酮升高的时间比青春期前男孩更早开始。在青春期早期和中期发现昼夜节律最为明显。
结论：使用一种灵敏的方法以及对青春期的重新分类，我们确定了青春期前和青春期男孩睾酮和计算游离睾酮的比较水平。比较数据的存在为未来青春期疾病的研究奠定了基础。
Objective: To establish levels for comparison for 24-h total and free serum testosterone in prepubertal boys and throughout pubertal development. Design: The study subjects were 55 healthy boys, aged 5.0-18.6 years, who underwent serial sampling one or more times during their pubertal development.Methods: Testicular volumes were determined by orchidometer. Serum testosterone was measured by a modified RIA (detection limit, 0.03 nmol/l). Free testosterone was calculated (calc-FT) using a formula derived from the law of mass action.Results: Significant increases in testosterone and calc-FT concentrations in boys were found between testis volumes of 1 ml to 2 ml, 2 ml to 3 ml, 6 ml to 8 ml, and 10 in[ to 15 ml. No differences were found between testis volumes of 3, 4, 5 and 6 ml neither were there differences between 8 and 10 nil, or between 15, 2 0 and 2 5 nil. Boys who had reached their final height had higher calc-FT values than boys who had the same pubertal development but had not reached their final height. Based on the results, puberty was classified into six stages: pre1 (testis, 1 ml), pre2 (testis, 2 ml), early (testis, 3-6ml), mid (testis, 8-12 ml), late1 (testis,15-25m]. not reached final height) and late2 (testis, 15-25ml, reached final height). Serum testosterone was secreted with a diurnal variation in prepuberty and during puberty. The increase of testosterone in the morning hours started earlier in pubertal than in pre-pubertal boys. The most pronounced diurnal rhythm was found in early and in mid puberty.Conclusion: Using a sensitive method. and a pubertal reclassification, we have established levels for comparison of testosterone and calc-FT in prepubertal and pubertal boys, The existence of data for comparison forms the basis for future studies on pubertal disorders.